High-throughput sequencing analysis revealed the regulation patterns of small RNAs on the development of A. comosus var. bracteatus leaves.
High-throughput sequencing analysis revealed the regulation patterns of small RNAs on the development of A. comosus var. bracteatus leaves.
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高通量测序分析揭示了小RNA对A. comosus var.发育的调控模式。
DOI：
10.1038/s41598-018-20261-z
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发表时间：
2018-01-31
影响因子：
4.6
通讯作者：
Sun LX
中科院分区：
文献类型：
--
作者：
Xiong YY;Ma J;He YH;Lin Z;Li X;Yu SM;Li RX;Jiang FX;Li X;Huang Z;Sun LX
研究白化病叶细胞形成的分子机制对于理解菠萝嵌合体叶的发育具有重要意义。具苞片的本研究利用高通量测序方法，共鉴定了163个参与完全白色（CWh）和完全绿色（CGr）叶片发育的新miRNAs。使用NR、Swiss-Prot、GO、COG、KEGG、KOG和Pfam数据库预测和注释潜在的miRNA靶基因。揭示了miRNAs调控的主要生物学过程。选择在CWh叶片细胞的发育和白化病中可能起重要作用的miRNAs并分析其表达模式。使用qRT-PCR研究9种miRNAs及其潜在靶基因的表达水平。这些结果将有助于阐明miRNAs在白化病细胞的形成和A.丛毛变种具苞片的这些数据也可能是有用的资源，为研究小RNA在其他叶色嵌合植物物种。
Studies of the molecular mechanisms involved in the formation of the albino leaf cells are important for understanding the development of chimera leaves in Ananas comosus var. bracteatus. In this study, we identified a total of 163 novel miRNAs involved in the development of complete white (CWh) and complete green (CGr) leaves using high-throughput sequencing method. The potential miRNA target genes were predicted and annotated using the NR, Swiss-Prot, GO, COG, KEGG, KOG and Pfam databases. The main biological processes regulated by miRNAs were revealed. The miRNAs which potentially play important roles in the development of the leaves and the albino of the CWh leaf cells were selected and their expression patterns were analyzed. The expression levels of nine miRNAs and their potential target genes were studied using qRT-PCR. These results will help to elucidate the functional and regulatory roles of miRNAs in the formation of the albino cells and the development of the leaves of A. comosus var. bracteatus. These data may also be helpful as a resource for studies of small RNA in other leaf color chimeric plant species.
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