High-throughput sequencing of small RNAs and anatomical characteristics associated with leaf development in celery.
High-throughput sequencing of small RNAs and anatomical characteristics associated with leaf development in celery.
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芹菜叶片发育相关小 RNA 和解剖特征的高通量测序
DOI：
10.1038/srep11093
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发表时间：
2015-06-09
影响因子：
4.6
通讯作者：
Xiong AS
中科院分区：
文献类型：
--
作者：
Jia XL;Li MY;Jiang Q;Xu ZS;Wang F;Xiong AS
MicroRNAs(MiRNAs)在植物的生长、发育和逆境反应中发挥着广泛而重要的作用，并在转录后水平上调节基因的表达。关于miRNAs的多样性及其在芹菜叶片发育中的作用的知识仍不清楚。为了阐明miRNAs在芹菜叶片发育中的作用，我们通过高通量测序鉴定了与叶片发育相关的miRNAs。以芹菜品种‘文图拉’三个时期(10、20和30 cm)的叶片为材料，构建了小分子文库，并进行了高通量测序和生物信息学分析。在‘Ventura’的阶段1、阶段2和阶段3，共鉴定了333、329和344个保守的miRNAs(分别属于35、35和32个家族)。在‘Ventura’中，共有131个miRNAs被鉴定为新的。使用eggnog、GO和KEGG数据库预测和注释潜在的miRNA靶基因，以探索基因功能。验证了5个保守的miRNAs及其对应的潜在靶基因的丰度。还检测了新的潜在miRNAs的表达谱。进一步分析了3个叶期叶片和叶柄的解剖特征。本研究有助于我们理解miRNAs在芹菜叶片发育过程中的功能和分子调控机制。
MicroRNAs (miRNAs) exhibit diverse and important roles in plant growth, development, and stress responses and regulate gene expression at the post-transcriptional level. Knowledge about the diversity of miRNAs and their roles in leaf development in celery remains unknown. To elucidate the roles of miRNAs in celery leaf development, we identified leaf development-related miRNAs through high-throughput sequencing. Small RNA libraries were constructed using leaves from three stages (10, 20, and 30 cm) of celery cv.‘Ventura’ and then subjected to high-throughput sequencing and bioinformatics analysis. At Stage 1, Stage 2, and Stage 3 of ‘Ventura’, a total of 333, 329, and 344 conserved miRNAs (belonging to 35, 35, and 32 families, respectively) were identified. A total of 131 miRNAs were identified as novel in ‘Ventura’. Potential miRNA target genes were predicted and annotated using the eggNOG, GO, and KEGG databases to explore gene functions. The abundance of five conserved miRNAs and their corresponding potential target genes were validated. Expression profiles of novel potential miRNAs were also detected. Anatomical characteristics of the leaf blades and petioles at three leaf stages were further analyzed. This study contributes to our understanding on the functions and molecular regulatory mechanisms of miRNAs in celery leaf development.
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毛果杨中 microRNA 相关铜调节网络的保守性和多样性。
影响因子：
11.4
作者：
Lu, Shanfa;Yang, Chenmin;Chiang, Vincent L.
通讯作者：
Chiang, Vincent L.
影响因子：
27.5
作者：
Bertolini E;Verelst W;Horner DS;Gianfranceschi L;Piccolo V;Inzé D;Pè ME;Mica E
通讯作者：
Mica E
影响因子：
30.8
作者：
Cullen, Bryan R.
通讯作者：
Cullen, Bryan R.
本地芹菜品种中小 RNA 的高通量分析以及受非生物胁迫影响的新型 microRNA 的表征
影响因子：
4.3
作者：
Jiang, Qian;Wang, Feng;Xiong, Ai-Sheng
通讯作者：
Xiong, Ai-Sheng
影响因子：
4.6
作者：
Berger, Yael;Harpaz-Saad, Smadar;Ori, Naomi
通讯作者：
Ori, Naomi