SSRP1 silencing inhibits the proliferation and malignancy of human glioma cells via the MAPK signaling pathway.
SSRP1 silencing inhibits the proliferation and malignancy of human glioma cells via the MAPK signaling pathway.
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SSRP1沉默通过MAPK信号通路抑制人胶质瘤细胞的增殖和恶性
DOI：
10.3892/or.2017.5982
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发表时间：
2017-11
期刊：
影响因子：
4.2
通讯作者：
Chen Q
中科院分区：
文献类型：
--
作者：
Liao J;Tao X;Ding Q;Liu J;Yang X;Yuan FE;Yang JA;Liu B;Xiang GA;Chen Q
结构特异性识别蛋白1（Structure-specific recognition protein 1，SSRP 1）在多种恶性肿瘤中异常高表达，被认为是一种潜在的生物标志物。然而，SSRP 1的表达水平与胶质瘤之间的相关性尚不清楚。本研究旨在探讨SSRP 1在胶质瘤发病中的作用。在本研究中，我们的数据显示，SSRP 1过表达检测胶质瘤组织中的mRNA和蛋白质水平，采用定量实时RT-PCR和免疫组化分析。我们还证明了SSRP 1的上调表达与世界卫生组织（WHO）胶质瘤分级相关。siRNA敲低SSRP 1基因不仅能抑制胶质瘤细胞的增殖，而且能显著抑制胶质瘤细胞的迁移和侵袭。机制分析表明，SSRP 1耗竭抑制MAPK信号通路的磷酸化活性。结论：SSRP 1通过MAPK信号通路调控胶质瘤细胞的增殖和转移。
Structure-specific recognition protein 1 (SSRP1) has been considered as a potential biomarker, since aberrant high expression of SSRP1 has been detected in numerous malignant tumors. However, the correlation between the expression level of SSRP1 and glioma remains unclear. The present study attempted to investigate the role of SSRP1 in the pathogenesis of glioma. In the present study, our data revealed that SSRP1 overexpression was detected in glioma tissues at both the mRNA and protein levels using quantitative real-time RT-PCR and immunohistochemical analysis. We also demonstrated that the upregulated expression of SSRP1 was correlated with the World Health Organization (WHO) grade of glioma. The knockdown of SSRP1 by siRNA not only resulted in the inhibition of cell proliferation, but also significantly inhibited glioma cell migration and invasion. Mechanistic analyses revealed that SSRP1 depletion suppressed the activity of the phosphorylation of the MAPK signaling pathway. In conclusion, the present study indicated that SSRP1 regulated the proliferation and metastasis of glioma cells via the MAPK signaling pathway.
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影响因子：
17.1
作者：
Carter DR;Murray J;Cheung BB;Gamble L;Koach J;Tsang J;Sutton S;Kalla H;Syed S;Gifford AJ;Issaeva N;Biktasova A;Atmadibrata B;Sun Y;Sokolowski N;Ling D;Kim PY;Webber H;Clark A;Ruhle M;Liu B;Oberthuer A;Fischer M;Byrne J;Saletta F;Thwe le M;Purmal A;Haderski G;Burkhart C;Speleman F;De Preter K;Beckers A;Ziegler DS;Liu T;Gurova KV;Gudkov AV;Norris MD;Haber M;Marshall GM
通讯作者：
Marshall GM
SSRP1 导致肝细胞癌的恶性，并受到 miR-497 的负调控。
DOI：
10.1038/mt.2016.9
发表时间：
2016-05
期刊：
Molecular therapy : the journal of the American Society of Gene Therapy
影响因子：
--
作者：
Ding Q;He K;Luo T;Deng Y;Wang H;Liu H;Zhang J;Chen K;Xiao J;Duan X;Huang R;Xia Z;Zhou W;He J;Yu H;Jiao X;Xiang G
通讯作者：
Xiang G
影响因子：
11
作者：
Tan BC;Liu H;Lin CL;Lee SC
通讯作者：
Lee SC
影响因子：
7.4
作者：
Shingu T;Holmes L;Henry V;Wang Q;Latha K;Gururaj AE;Gibson LA;Doucette T;Lang FF;Rao G;Yuan L;Sulman EP;Farrell NP;Priebe W;Hess KR;Wang YA;Hu J;Bögler O
通讯作者：
Bögler O
影响因子：
8.8
作者：
Garcia H;Miecznikowski JC;Safina A;Commane M;Ruusulehto A;Kilpinen S;Leach RW;Attwood K;Li Y;Degan S;Omilian AR;Guryanova O;Papantonopoulou O;Wang J;Buck M;Liu S;Morrison C;Gurova KV
通讯作者：
Gurova KV