Stem Cells and the Endometrium: From the Discovery of Adult Stem Cells to Pre-Clinical Models.
Stem Cells and the Endometrium: From the Discovery of Adult Stem Cells to Pre-Clinical Models.
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DOI：
10.3390/cells10030595
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发表时间：
2021-03-08
期刊：
影响因子：
6
通讯作者：
Cervelló I
中科院分区：
文献类型：
--
作者：
de Miguel-Gómez L;López-Martínez S;Francés-Herrero E;Rodríguez-Eguren A;Pellicer A;Cervelló I
成体干细胞(ASCs)一直被认为存在于子宫内膜。事实上，通过不同的分离和鉴定技术，在啮齿类动物和人类中鉴定出了几种类型的子宫内膜ASCs。利用标记保留技术在小鼠模型中鉴定了假定的基质干细胞和上皮干细胞的生态位。在人类中，功能性方法(克隆形成、长期培养和多谱系分化试验)和干细胞标记物(CD146、SUSD2/W5C5、LGR5、NTPDase2、SSEA-1或N-cadherin)有助于鉴定三种主要类型的内源性子宫内膜ASCs：间质干细胞、上皮祖细胞和内皮干细胞。此外，来自骨髓的外源性干细胞群体可能是子宫内膜ASC生态位的关键效应者。这些发现正在推动干细胞疗法治疗子宫内膜病变的发展，并向旁分泌方法发展。与此同时，基于生物工程的治疗方案也被提出了很有前景的替代方案。
Adult stem cells (ASCs) were long suspected to exist in the endometrium. Indeed, several types of endometrial ASCs were identified in rodents and humans through diverse isolation and characterization techniques. Putative stromal and epithelial stem cell niches were identified in murine models using label-retention techniques. In humans, functional methods (clonogenicity, long-term culture, and multi-lineage differentiation assays) and stem cell markers (CD146, SUSD2/W5C5, LGR5, NTPDase2, SSEA-1, or N-cadherin) facilitated the identification of three main types of endogenous endometrial ASCs: stromal, epithelial progenitor, and endothelial stem cells. Further, exogenous populations of stem cells derived from bone marrow may act as key effectors of the endometrial ASC niche. These findings are promoting the development of stem cell therapies for endometrial pathologies, with an evolution towards paracrine approaches. At the same time, promising therapeutic alternatives based on bioengineering have been proposed.
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