Insights into the preservation of the homomorphic sex-determining chromosome of Aedes aegypti from the discovery of a male-biased gene tightly linked to the M-locus.
Insights into the preservation of the homomorphic sex-determining chromosome of Aedes aegypti from the discovery of a male-biased gene tightly linked to the M-locus.
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DOI：
10.1093/gbe/evu002
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发表时间：
2014-01
影响因子：
3.3
通讯作者：
Tu Z
中科院分区：
文献类型：
--
作者：
Hall AB;Timoshevskiy VA;Sharakhova MV;Jiang X;Basu S;Anderson MA;Hu W;Sharakhov IV;Adelman ZN;Tu Z
在某些生物体中，决定性别的同型染色体能够保存下来而不转化为异型性染色体，这是进化生物学中一个长期存在的谜。在埃及伊蚊中，未分化的同形染色体上的显性性别决定基因座（或M基因座）赋予了雄性表型。遗传学证据表明，M位点是在一个nonrecombining区。然而，M基因座的分子性质尚未被表征。使用最近开发的基于Illumina对男性和女性基因组DNA测序的方法，我们确定了一种新的基因myo-sex，该基因几乎完全存在于男性基因组中，但由于重组，可以偶尔在女性基因组中发现。为了简单起见，我们将主要在男性基因组中发现的序列定义为男性偏好的。荧光原位杂交（FISH）技术检测A.埃及人的染色体表明，myo-sex探针定位于区域1 q21，即M基因座的确定位置。Myo-sex是一个重复的肌球蛋白重链基因，在蛹和成年雄性中高度表达。Myo-sex与其最接近的常染色体同源序列具有83%的核苷酸同一性和97%的氨基酸同一性，这与古老的重复以及随后的强纯化选择一致。与男性相比，myo-sex在获得它的女性中的表达水平非常低，这表明myo-sex可能是性对抗的。这项研究建立了一个框架，发现男性偏向的序列内的一个同态性别决定染色体，并提供了新的见解的进化力量，阻碍了扩展的nonrecombining M-基因座在A。埃及人。
The preservation of a homomorphic sex-determining chromosome in some organisms without transformation into a heteromorphic sex chromosome is a long-standing enigma in evolutionary biology. A dominant sex-determining locus (or M-locus) in an undifferentiated homomorphic chromosome confers the male phenotype in the yellow fever mosquito Aedes aegypti. Genetic evidence suggests that the M-locus is in a nonrecombining region. However, the molecular nature of the M-locus has not been characterized. Using a recently developed approach based on Illumina sequencing of male and female genomic DNA, we identified a novel gene, myo-sex, that is present almost exclusively in the male genome but can sporadically be found in the female genome due to recombination. For simplicity, we define sequences that are primarily found in the male genome as male-biased. Fluorescence in situ hybridization (FISH) on A. aegypti chromosomes demonstrated that the myo-sex probe localized to region 1q21, the established location of the M-locus. Myo-sex is a duplicated myosin heavy chain gene that is highly expressed in the pupa and adult male. Myo-sex shares 83% nucleotide identity and 97% amino acid identity with its closest autosomal paralog, consistent with ancient duplication followed by strong purifying selection. Compared with males, myo-sex is expressed at very low levels in the females that acquired it, indicating that myo-sex may be sexually antagonistic. This study establishes a framework to discover male-biased sequences within a homomorphic sex-determining chromosome and offers new insights into the evolutionary forces that have impeded the expansion of the nonrecombining M-locus in A. aegypti.
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影响因子：
2.6
作者：
Criscione F;Qi Y;Saunders R;Hall B;Tu Z
通讯作者：
Tu Z
DOI：
10.1098/rstb.2000.0717
发表时间：
2000-11-29
影响因子：
6.3
作者：
Charlesworth, B;Charlesworth, D
通讯作者：
Charlesworth, D
影响因子：
3.8
作者：
Adelman, Zach N.;Anderson, Michelle A. E.;Myles, Kevin M.
通讯作者：
Myles, Kevin M.
影响因子：
7
作者：
Ferdig, MT;Taft, AS;Christensen, BM
通讯作者：
Christensen, BM
影响因子：
4.5
作者：
Comeron JM;Ratnappan R;Bailin S
通讯作者：
Bailin S