Influences of membrane mimetic environments on membrane protein structures.
Influences of membrane mimetic environments on membrane protein structures.
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DOI：
10.1146/annurev-biophys-083012-130326
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发表时间：
2013
影响因子：
12.4
通讯作者：
Cross TA
中科院分区：
文献类型：
--
作者：
Zhou HX;Cross TA
蛋白质数据库中的膜蛋白结构的数量正变得越来越重要。在这里，螺旋膜蛋白的跨膜结构域的结构进行评估，以评估膜模拟环境的影响。为了实现这一目标，许多膜的生物物理性质进行了讨论，并与通常用于结构测定的膜模拟物进行对比。虽然模拟环境可以扰乱蛋白质结构的程度，可能会引起误解的功能机制，也有许多结构，有一个天然的外观。从这一评估，提出了一套初步的指导方针，用于区分天然样非天然样膜蛋白结构。随着实验技术的验证和计算方法的细化和质量评估和增强，有很好的前景，实现这些非常重要的蛋白质的天然样结构。
The number of membrane protein structures in the Protein Data Bank is becoming significant and growing. Here, the transmembrane domain structures of the helical membrane proteins are evaluated to assess the influences of the membrane mimetic environments. Toward this goal, many of the biophysical properties of membranes are discussed and contrasted with those of the membrane mimetics commonly used for structure determination. Although the mimetic environments can perturb the protein structures to an extent that potentially gives rise to misinterpretation of functional mechanisms, there are also many structures that have a native-like appearance. From this assessment, an initial set of guidelines is proposed for distinguishing native-like from nonnative-like membrane protein structures. With experimental techniques for validation and computational methods for refinement and quality assessment and enhancement, there are good prospects for achieving native-like structures for these very important proteins.
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影响因子：
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通讯作者：
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