Physicochemical characterization and genotoxicity of the broad class of carbon nanotubes and nanofibers used or produced in U.S. facilities.
Physicochemical characterization and genotoxicity of the broad class of carbon nanotubes and nanofibers used or produced in U.S. facilities.
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DOI：
10.1186/s12989-020-00392-w
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发表时间：
2020-12-07
影响因子：
10
通讯作者：
Erdely A
中科院分区：
文献类型：
--
作者：
Fraser K;Kodali V;Yanamala N;Birch ME;Cena L;Casuccio G;Bunker K;Lersch TL;Evans DE;Stefaniak A;Hammer MA;Kashon ML;Boots T;Eye T;Hubczak J;Friend SA;Dahm M;Schubauer-Berigan MK;Siegrist K;Lowry D;Bauer AK;Sargent LM;Erdely A
碳纳米管和纳米纤维（CNT/F）具有已知的毒性，但缺乏对广泛材料类别（特别是直径较大的材料）的同时比较研究，以及将毒性与其基本材料特性联系起来的计算分析。尚不清楚所有CNT/F是否具有相似的毒性，特别是遗传毒性。对美国机构暴露评估研究中常见的9种CNT/F（MW#1 -7和CNF#1 -2）进行了评价，报告的直径范围为6 - 150 nm。对所有材料进行了广泛表征，包括物理尺寸分布和成束附聚物的普遍性。将人支气管上皮细胞暴露于9种CNT/F（0-24 μg/ml），以测定细胞活力、炎症、细胞氧化应激、微核形成和DNA双链断裂。计算建模用于了解理化特性和毒性结果的各种排列。CNT/F物理化学特性的分析表明，与单独使用颗粒尺寸平均值相比，使用物理尺寸的详细分布提供了更一致的CNT/F分组。事实上，单独对标称管物理尺寸进行分组的分析产生了与所有表征参数一起类似的分组。所有材料均在试验剂量范围内诱导上皮细胞毒性和微核形成。细胞氧化应激、DNA双链断裂和微核形成始终聚集在一起，并具有较大的物理CNT/F尺寸和聚集特征，但与炎性蛋白变化不同。较大的标称管直径、较长的长度和成束的团聚物特征与较大的影响严重度相关。样品中标称长度和直径较大的管部分在数量上并不占多数，这意味着具有这些特征的管的较小百分比足以增加毒性。许多传统的物理化学特征，包括表面积，密度，杂质，和灰尘没有集群的毒性结果。与仅平均值相比，物理尺寸的分布提供了更一致的CNT/F毒性结局分组。所有CNT/F均在人上皮细胞中诱导一定水平的遗传毒性。毒性的严重程度取决于样品中含有一定比例的标称长度和直径较大的管。
Carbon nanotubes and nanofibers (CNT/F) have known toxicity but simultaneous comparative studies of the broad material class, especially those with a larger diameter, with computational analyses linking toxicity to their fundamental material characteristics was lacking. It was unclear if all CNT/F confer similar toxicity, in particular, genotoxicity. Nine CNT/F (MW #1–7 and CNF #1–2), commonly found in exposure assessment studies of U.S. facilities, were evaluated with reported diameters ranging from 6 to 150 nm. All materials were extensively characterized to include distributions of physical dimensions and prevalence of bundled agglomerates. Human bronchial epithelial cells were exposed to the nine CNT/F (0–24 μg/ml) to determine cell viability, inflammation, cellular oxidative stress, micronuclei formation, and DNA double-strand breakage. Computational modeling was used to understand various permutations of physicochemical characteristics and toxicity outcomes. Analyses of the CNT/F physicochemical characteristics illustrate that using detailed distributions of physical dimensions provided a more consistent grouping of CNT/F compared to using particle dimension means alone. In fact, analysis of binning of nominal tube physical dimensions alone produced a similar grouping as all characterization parameters together. All materials induced epithelial cell toxicity and micronuclei formation within the dose range tested. Cellular oxidative stress, DNA double strand breaks, and micronuclei formation consistently clustered together and with larger physical CNT/F dimensions and agglomerate characteristics but were distinct from inflammatory protein changes. Larger nominal tube diameters, greater lengths, and bundled agglomerate characteristics were associated with greater severity of effect. The portion of tubes with greater nominal length and larger diameters within a sample was not the majority in number, meaning a smaller percentage of tubes with these characteristics was sufficient to increase toxicity. Many of the traditional physicochemical characteristics including surface area, density, impurities, and dustiness did not cluster with the toxicity outcomes. Distributions of physical dimensions provided more consistent grouping of CNT/F with respect to toxicity outcomes compared to means only. All CNT/F induced some level of genotoxicity in human epithelial cells. The severity of toxicity was dependent on the sample containing a proportion of tubes with greater nominal lengths and diameters.
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影响因子：
4.9
作者：
Bahl, Aileen;Hellack, Bryan;Haase, Andrea
通讯作者：
Haase, Andrea
DOI：
10.1016/j.ijheh.2020.113472
发表时间：
2020-04-01
影响因子：
6
作者：
Canu, I. Guseva;Batsungnoen, K.;Hopf, N. B.
通讯作者：
Hopf, N. B.
DOI：
10.1080/02786826.2019.1618437
发表时间：
2019
期刊：
Aerosol science and technology : the journal of the American Association for Aerosol Research
影响因子：
--
作者：
Dahm MM;Evans DE;Bertke S;Grinshpun SA
通讯作者：
Grinshpun SA
影响因子：
--
作者：
Dahm, Matthew M.;Schubauer-Berigan, Mary K.;Deddens, James A.
通讯作者：
Deddens, James A.
影响因子：
5
作者：
Bannuscher, Anne;Karkossa, Isabel;Schubert, Kristin
通讯作者：
Schubert, Kristin