Targeted multimodal nano-reporters for pre-procedural MRI and intra-operative image-guidance.
Targeted multimodal nano-reporters for pre-procedural MRI and intra-operative image-guidance.
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DOI：
10.1016/j.biomaterials.2016.09.013
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发表时间：
2016-12
期刊：
影响因子：
14
通讯作者：
Kim DH
中科院分区：
文献类型：
--
作者：
Lee J;Gordon AC;Kim H;Park W;Cho S;Lee B;Larson AC;Rozhkova EA;Kim DH
多模态成像探针提供了一种新的方法，可以为临床实践中图像引导治疗的规划提供详细的诊断信息。在这里，我们报道了靶向多模态掺杂Nd3+的上转换纳米颗粒（UCNP）成像报告，将磁共振成像（MRI）和实时上转换发光成像（UCL）功能集成在一个单一平台内。合成的Nd3+掺杂UCNPs为核壳结构，在近红外激发下显示明亮的可见发射（最大限度地减少生物过热并增加组织穿透深度），并提供强大的MRI T2对比度（高r2/r1比）。经导管动脉内灌注Nd3+掺杂与抗cd44单克隆抗体结合的UCNPs，可以在原位大鼠模型中实现高效的体内多模态UCL和肝细胞癌（HCC）的MR成像。结果表明，Nd3+掺核-壳UCNPs的体内多模态成像结合经导管动脉内靶向方法成功地将大鼠肝脏肿瘤与正常肝脏组织区分开来，用于手术切除。我们的多模态UCL和多模态UCNPs报告者的MRI成像能力表明，在肿瘤的体内可视化和精确的手术指导方面具有强大的潜力，可以填补术前成像和术中现实之间的空白。
Multimodal-imaging probes offer a novel approach, which can provide detail diagnostic information for the planning of image-guided therapies in clinical practice. Here we report targeted multimodal Nd3+-doped upconversion nanoparticle (UCNP) imaging reporters, integrating both magnetic resonance imaging (MRI) and real-time upconversion luminescence imaging (UCL) capabilities within a single platform. Nd3+-doped UCNPs were synthesized as a core–shell structure showing a bright visible emission upon excitation at the near infrared (minimizing biological overheating and increasing tissue penetration depth) as well as providing strong MRI T2 contrast (high r2/r1 ratio). Transcatheter intra-arterial infusion of Nd3+-doped UCNPs conjugated with anti-CD44-monoclonal antibody allowed for high performance in vivo multimodal UCL and MR imaging of hepatocellular carcinoma (HCC) in an orthotopic rat model. The resulted in vivo multimodal imaging of Nd3+ doped core-shell UCNPs combined with transcatheter intra-arterial targeting approaches successfully discriminated liver tumors from normal hepatic tissues in rats for surgical resection applications. The demonstrated multimodal UCL and MRI imaging capabilities of our multimodal UCNPs reporters suggest strong potential for in vivo visualization of tumors and precise surgical guidance to fill the gap between pre-procedural imaging and intraoperative reality.
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荧光引导的手术和实时导航 - 一种新的尖端。
DOI：
10.1038/nrc3566
发表时间：
2013-09
期刊：
Nature reviews. Cancer
影响因子：
--
作者：
通讯作者：
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上转换纳米颗粒：设计、纳米化学及其在治疗诊断学中的应用
影响因子：
62.1
作者：
Chen, Guanying;Qju, Hailong;Prasad, Paras N.;Chen, Xiaoyuan
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影响因子：
14
作者：
Chen, Jeane;White, Sarah B.;Harris, Kathleen R.;Li, Weiguo;Yap, Jonathan W. T.;Kim, Dong-Hyun;Lewandowski, Robert J.;Shea, Lonnie D.;Larson, Andrew C.
通讯作者：
Larson, Andrew C.
影响因子：
5.6
作者：
Bullivant JP;Zhao S;Willenberg BJ;Kozissnik B;Batich CD;Dobson J
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影响因子：
44.1
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Hou, Ying;Zou, Qifei;Wang, Yizheng
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Wang, Yizheng