Acoustofluidics for simultaneous nanoparticle-based drug loading and exosome encapsulation.
Acoustofluidics for simultaneous nanoparticle-based drug loading and exosome encapsulation.
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DOI：
10.1038/s41378-022-00374-2
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发表时间：
2022
影响因子：
7.9
通讯作者：
Huang, Tony Jun
中科院分区：
文献类型：
--
作者：
Wang, Zeyu;Rich, Joseph;Hao, Nanjing;Gu, Yuyang;Chen, Chuyi;Yang, Shujie;Zhang, Peiran;Huang, Tony Jun
已经发现纳米载体和外泌体包封显著增加靶向药物递送的功效，同时还最小化不想要的副作用。然而，外泌体包封的药物纳米载体的开发受到低载药效率和/或复杂、耗时的载药过程的限制。在此，我们已经开发了一种声流体装置，其同时进行药物装载和外泌体包封。通过协同地利用旋转液滴中的声辐射力、声微流和剪切应力，实现了外泌体、药物和多孔二氧化硅纳米颗粒的浓缩和融合。最终产品由包裹在外泌体膜内的载药二氧化硅纳米载体组成。载药效率显著提高，近30%的游离药物（例如，阿霉素）分子。此外，这种声流体药物装载系统避免了复杂的化学修饰的需要，允许药物装载和封装在几分钟内完成。这些外泌体包封的纳米载体在细胞内转运中表现出优异的效率，并且能够显著抑制肿瘤细胞增殖。通过利用物理力快速生成混合纳米载体，这种声流体药物加载平台具有显著影响药物递送研究和应用创新的潜力。
Nanocarrier and exosome encapsulation has been found to significantly increase the efficacy of targeted drug delivery while also minimizing unwanted side effects. However, the development of exosome-encapsulated drug nanocarriers is limited by low drug loading efficiencies and/or complex, time-consuming drug loading processes. Herein, we have developed an acoustofluidic device that simultaneously performs both drug loading and exosome encapsulation. By synergistically leveraging the acoustic radiation force, acoustic microstreaming, and shear stresses in a rotating droplet, the concentration, and fusion of exosomes, drugs, and porous silica nanoparticles is achieved. The final product consists of drug-loaded silica nanocarriers that are encased within an exosomal membrane. The drug loading efficiency is significantly improved, with nearly 30% of the free drug (e.g., doxorubicin) molecules loaded into the nanocarriers. Furthermore, this acoustofluidic drug loading system circumvents the need for complex chemical modification, allowing drug loading and encapsulation to be completed within a matter of minutes. These exosome-encapsulated nanocarriers exhibit excellent efficiency in intracellular transport and are capable of significantly inhibiting tumor cell proliferation. By utilizing physical forces to rapidly generate hybrid nanocarriers, this acoustofluidic drug loading platform wields the potential to significantly impact innovation in both drug delivery research and applications.
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影响因子：
4.6
作者：
Gong, Zhixiong;Baudoin, Michael
通讯作者：
Baudoin, Michael
影响因子：
4.6
作者：
Cox, Luke;Melde, Kai;Drinkwater, Bruce W.
通讯作者：
Drinkwater, Bruce W.
DOI：
10.1073/pnas.1917125117
发表时间：
2020-05-19
影响因子：
11.1
作者：
Belling, Jason N.;Heidenreich, Liv K.;Jonas, Steven J.
通讯作者：
Jonas, Steven J.
影响因子：
16.1
作者：
Cai SS;Li T;Akinade T;Zhu Y;Leong KW
通讯作者：
Leong KW
影响因子：
5.4
作者：
Antimisiaris SG;Mourtas S;Marazioti A
通讯作者：
Marazioti A