Biohybrid Membrane Formation by Directed Insertion of Aquaporin into a Solid-State Nanopore.
Biohybrid Membrane Formation by Directed Insertion of Aquaporin into a Solid-State Nanopore.
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DOI：
10.1021/acsami.2c14250
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发表时间：
2022-10-26
影响因子：
9.5
通讯作者：
Yazaydin, A. Ozgur
中科院分区：
文献类型：
--
作者：
Sicard, Francois;Yazaydin, A. Ozgur
生物杂化纳米孔联合收割机将固态纳米孔的耐久性与生物纳米孔的精确结构和功能相结合。必须特别注意控制生物纳米孔一旦与固态纳米孔接触就如何适应其周围环境。两个主要挑战是在动态条件下精确控制这种适应性，并提供可用于工程应用的预设计功能。在这项工作中，我们报告了一类独特的生物混合活性膜层的计算设计，从直接插入的水通道蛋白纳入脂质nanodisc模型烷基官能化的二氧化硅孔。我们表明，在水性环境中，当固态纳米孔的两侧之间存在压力差时，围绕水通道蛋白的脂质分子的烃尾和使二氧化硅纳米孔的内表面官能化的烷基之间的优先相互作用使水通道蛋白掺入的脂质壳能够通过挤出水分子插入到纳米孔中。相同的优先相互作用负责插入的水通道蛋白掺入的脂质壳的结构稳定性以及脂质-烷基界面的水密封性质。我们进一步表明，稳定在烷基官能化的二氧化硅纳米孔中的水通道蛋白在纯水和盐水中保留了其生物结构和功能，并且值得注意的是，其水渗透性等于在生物环境中测量的水渗透性。所设计的生物混合膜可以为开发用于水过滤的耐用变革设备铺平道路。
Biohybrid nanopores combine the durability of solid-state nanopores with the precise structure and function of biological nanopores. Particular care must be taken to control how biological nanopores adapt to their surroundings once they come into contact with the solid-state nanopores. Two major challenges are to precisely control this adaptability under dynamic conditions and provide predesigned functionalities that can be manipulated for engineering applications. In this work, we report on the computational design of a distinctive class of biohybrid active membrane layers, built from the directed-insertion of an aquaporin-incorporated lipid nanodisc into a model alkyl-functionalized silica pore. We show that in an aqueous environment when a pressure difference exists between the two sides of the solid-state nanopore, the preferential interactions between the hydrocarbon tail of the lipid molecules that surround the aquaporin protein and the alkyl group functionalizing the interior surface of the silica nanopore enable the insertion of the aquaporin-incorporated lipid shell into the nanopore by forcing out the water molecules. The same preferential interactions are responsible for the structural stability of the inserted aquaporin-incorporated lipid shell as well as the water sealing properties of the lipid–alkyl interface. We further show that the aquaporin protein stabilized in the alkyl-functionalized silica nanopore preserves its biological structure and function in both pure and saline water, and, remarkably, its water permeability is equal to the one measured in the biological environment. The designed biohybrid membrane could pave the way for the development of durable transformative devices for water filtration.
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DOI：
10.3390/nano8040237
发表时间：
2018-04-13
期刊：
Nanomaterials (Basel, Switzerland)
影响因子：
--
作者：
Farajollahi F;Seidenstücker A;Altintoprak K;Walther P;Ziemann P;Plettl A;Marti O;Wege C;Gliemann H
通讯作者：
Gliemann H
影响因子：
38.3
作者：
通讯作者：
--
DOI：
10.1073/pnas.1522034113
发表时间：
2016-04-19
影响因子：
11.1
作者：
Cullum, Jodie;Stevens, David P.;Joshi, Manoj M.
通讯作者：
Joshi, Manoj M.
影响因子：
9.9
作者：
Fuwad, Ahmed;Ryu, Hyunil;Jeon, Tae-Joon
通讯作者：
Jeon, Tae-Joon
使用麦芽糖结合蛋白/多组氨酸双亲和标签融合系统在大肠杆菌中高水平生产水通道蛋白 Z
影响因子：
4.4
作者：
Hang, Baojian;Pan, Jianfeng;Xu, Zhinan
通讯作者：
Xu, Zhinan