Electrochemically-Driven Insertion of Biological Nanodiscs into Solid State Membrane Pores as a Basis for "Pore-In-Pore" Membranes.
Electrochemically-Driven Insertion of Biological Nanodiscs into Solid State Membrane Pores as a Basis for "Pore-In-Pore" Membranes.
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DOI：
10.3390/nano8040237
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发表时间：
2018-04-13
期刊：
影响因子：
--
通讯作者：
Gliemann H
中科院分区：
文献类型：
--
作者：
Farajollahi F;Seidenstücker A;Altintoprak K;Walther P;Ziemann P;Plettl A;Marti O;Wege C;Gliemann H
纳米多孔膜在分子过滤器、生物传感器、纳米流体逻辑和能量转换装置等领域的应用越来越受到人们的关注。为了达到高质量的标准，例如，在分子分离过程中，需要在孔径和化学性质方面具有良好定义的孔的膜。然而，制备具有窄孔径分布的膜仍然具有挑战性。在这里的工作中，我们展示了一种策略，一种“孔中孔”的方法，在这种方法中，由多步骤自上而下的光刻工艺产生的固体膜的锥形孔被用作模板，以插入精确形成的具有精确定义的内径和外径的生物分子纳米片。这些纳米圆盘是烟草花叶病毒推断颗粒的基本组成部分，由外壳蛋白组成，它们在确定的实验条件下与稳定的短RNA自组装。我们证明了纳米片的插入既可以由浓度梯度引起的扩散驱动，也可以通过沿着固体膜的横截面施加电场驱动。结果表明，电泳驱动插入比浓度梯度插入更有效。
Nanoporous membranes are of increasing interest for many applications, such as molecular filters, biosensors, nanofluidic logic and energy conversion devices. To meet high-quality standards, e.g., in molecular separation processes, membranes with well-defined pores in terms of pore diameter and chemical properties are required. However, the preparation of membranes with narrow pore diameter distributions is still challenging. In the work presented here, we demonstrate a strategy, a “pore-in-pore” approach, where the conical pores of a solid state membrane produced by a multi-step top-down lithography procedure are used as a template to insert precisely-formed biomolecular nanodiscs with exactly defined inner and outer diameters. These nanodiscs, which are the building blocks of tobacco mosaic virus-deduced particles, consist of coat proteins, which self-assemble under defined experimental conditions with a stabilizing short RNA. We demonstrate that the insertion of the nanodiscs can be driven either by diffusion due to a concentration gradient or by applying an electric field along the cross-section of the solid state membrane. It is found that the electrophoresis-driven insertion is significantly more effective than the insertion via the concentration gradient.
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影响因子：
64.8
作者：
通讯作者：
--
影响因子：
38.3
作者：
通讯作者：
--
DOI：
10.1016/s0168-583x(01)00722-4
发表时间：
2001-11-01
影响因子：
1.3
作者：
Apel, PY;Korchev, YE;Yoshida, M
通讯作者：
Yoshida, M
影响因子：
10.8
作者：
James T;Kalinin YV;Chan CC;Randhawa JS;Gaevski M;Gracias DH
通讯作者：
Gracias DH
影响因子：
64.8
作者：
Li, H;Eddaoudi, M;Yaghi, OM
通讯作者：
Yaghi, OM