An RNA aptamer perturbs heat shock transcription factor activity in Drosophila melanogaster.
An RNA aptamer perturbs heat shock transcription factor activity in Drosophila melanogaster.
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DOI：
10.1093/nar/gkr206
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发表时间：
2011-08
影响因子：
14.9
通讯作者：
Lis JT
中科院分区：
文献类型：
--
作者：
Salamanca HH;Fuda N;Shi H;Lis JT
Heat shock transcription factor (HSF1) is a conserved master regulator that orchestrates the protection of normal cells from stress. However, HSF1 also protects abnormal cells and is required for carcinogenesis. Here, we generate an highly specific RNA aptamer (iaRNAHSF1) that binds Drosophila HSF1 and inhibits HSF1 binding to DNA. In Drosophila animals, iaRNAHSF1 reduces normal Hsp83 levels and promotes developmental abnormalities, mimicking the spectrum of phenotypes that occur when Hsp83 activity is reduced. The HSF1 aptamer also effectively suppresses the abnormal growth phenotypes induced by constitutively active forms of the EGF receptor and Raf oncoproteins. Our results indicate that HSF1 contributes toward the morphological development of animal traits by controlling the expression of molecular chaperones under normal growth conditions. Additionally, our study demonstrates the utility of the RNA aptamer technology as a promising chemical genetic approach to investigate biological mechanisms, including cancer and for identifying effective drug targets in vivo.
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影响因子：
11.4
作者：
Lange, BMH;Bachi, A;González, C
通讯作者：
González, C
DOI：
10.1073/pnas.88.16.6906
发表时间：
1991-08-01
影响因子：
11.1
作者：
RABINDRAN, SK;GIORGI, G;WU, C
通讯作者：
WU, C
影响因子：
64.5
作者：
Dai, Chengkai;Whitesell, Luke;Lindquist, Susan
通讯作者：
Lindquist, Susan
影响因子：
11.2
作者：
Sawai, Ayana;Chandarlapaty, Sarat;Solit, David B.
通讯作者：
Solit, David B.
影响因子：
64.5
作者：
Kang, Byoung Heon;Plescia, Janet;Altieri, Dario C.
通讯作者：
Altieri, Dario C.