Isolation and characterization of hexokinase genes PsHXK1 and PsHXK2 from tree peony (Paeonia suffruticosa Andrews)
Isolation and characterization of hexokinase genes PsHXK1 and PsHXK2 from tree peony (Paeonia suffruticosa Andrews)
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牡丹 (Paeonia suffruticosa Andrews) 己糖激酶基因 PsHXK1 和 PsHXK2 的分离和鉴定
DOI：
10.1007/s11033-019-05135-5
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发表时间：
2019-11
影响因子：
2.8
通讯作者：
董丽
中科院分区：
文献类型：
--
作者：
张超;张丽丽;付建新;董丽
己糖激酶（HXK）在己糖磷酸化和糖信号转导中起重要作用。HXK在许多物种中调节葡萄糖诱导的花青素苷积累。HXK基因家族的生物学功能目前尚不清楚。利用RACE-PCR和RT-PCR技术从木霉菌中克隆了两个己糖激酶基因PsHXK 1和PsHXK 2的cDNA序列。PsHXK 1编码498个氨基酸，开放阅读框（ORF）长度为1497-bp; PsHXK 2编码493个氨基酸，开放阅读框长度为1482-bp。序列分析和系统发育分析表明，PsHXK 1和PsHXK 2属于B型HXK，可能具有葡萄糖传感器的功能。PsHXK 1和PsHXK 2 mRNA在所有受试组织中均有表达。PsHXK 1在花瓣和雄蕊中高表达，而PsHXK 2在雄蕊中高表达。PsHXK 1和PsHXK 2在开花前期的树上花中的表达量高于切花。在拟南芥中过表达PsHXK 1和PsHXK 2增强葡萄糖敏感性，抑制植物响应于葡萄糖的生长，并诱导响应于高水平葡萄糖的花青素积累。总之，我们的研究结果初步揭示了PsHXK 1和PsHXK 2的生物学功能，特别是它们参与葡萄糖诱导的花青素积累。
Hexokinase (HXK) plays important roles in hexose phosphorylation and sugar signaling. HXK regulates the glucose-induced accumulation of anthocyanin in many species. Little is known about the biological function of the HXK gene family in Paeonia suffruticosa. cDNA sequences of two hexokinase genes PsHXK1 and PsHXK2 were isolated using RACE-PCR and RT-PCR from P. suffruticosa. PsHXK1 encodes 498 amino acids with a 1497-bp open reading frame (ORF), and PsHXK2 contains 493 amino acids with a 1482-bp ORF. Sequence and phylogenetic analyses suggest that PsHXK1 and PsHXK2 belong to type-B HXK and may function as glucose sensors. PsHXK1 and PsHXK2 mRNA were detected in all tested tissues. PsHXK1 is highly expressed in petals and stamens, while PsHXK2 is highly expressed in stamens. At the former stages of flower opening, PsHXK1 and PsHXK2 show higher expression levels in on-tree flowers compared with cut flowers. Overexpressing PsHXK1 and PsHXK2 in Arabidopsis enhances glucose sensitivity, inhibits plant growth in response to glucose, and induces anthocyanin accumulation in response to the high level of glucose. Overall, our results primarily reveal the biological function of PsHXK1 and PsHXK2, especially their involvement in glucose-induced anthocyanin accumulation.
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影响因子：
7.3
作者：
Kim, Young-Min;Heinzel, Nicolas;Hajirezaei, Mohammad-Reza
通讯作者：
Hajirezaei, Mohammad-Reza
影响因子：
4.3
作者：
Jingxue Wang;Xiaomin Wang;Siyu Geng;S. Singh;Yaohui Wang;Sitakanta Pattanaik;Ling Yuan
通讯作者：
Jingxue Wang;Xiaomin Wang;Siyu Geng;S. Singh;Yaohui Wang;Sitakanta Pattanaik;Ling Yuan
DOI：
--
发表时间：
2009
期刊：
--
影响因子：
--
作者：
Jung-Il Cho;Nayeon Ryoo;J. Eom;Dae-Woo Lee;Hyun-Bi Kim;Seok-Won Jeong;Youn-Hyung Lee;Yong-Kook Kwon;M. Cho;S. Bhoo;T. Hahn;Youn-Il Park;Ildoo Hwang;J. Sheen;J. Jeon
通讯作者：
Jung-Il Cho;Nayeon Ryoo;J. Eom;Dae-Woo Lee;Hyun-Bi Kim;Seok-Won Jeong;Youn-Hyung Lee;Yong-Kook Kwon;M. Cho;S. Bhoo;T. Hahn;Youn-Il Park;Ildoo Hwang;J. Sheen;J. Jeon
影响因子：
4.3
作者：
Jingxue Wang;Xiaomin Wang;Siyu Geng;S. Singh;Yaohui Wang;Sitakanta Pattanaik;Ling Yuan
通讯作者：
Jingxue Wang;Xiaomin Wang;Siyu Geng;S. Singh;Yaohui Wang;Sitakanta Pattanaik;Ling Yuan
影响因子：
7
作者：
Zhang, Chao;Wang, Weining;Dong, Li
通讯作者：
Dong, Li