Molecular characterization, expression and functional analysis of acyl-CoA-binding protein gene family in maize (Zea mays).
Molecular characterization, expression and functional analysis of acyl-CoA-binding protein gene family in maize (Zea mays).
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玉米酰基辅酶A结合蛋白基因家族的分子特征、表达和功能分析
DOI：
10.1186/s12870-021-02863-4
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发表时间：
2021-02-15
影响因子：
5.3
通讯作者：
Li H
中科院分区：
文献类型：
--
作者：
Zhu J;Li W;Zhou Y;Pei L;Liu J;Xia X;Che R;Li H
酰基辅酶A结合蛋白（Acyl-CoA-binding proteins，ACBPs）具有一个保守的酰基辅酶A结合（acyl-CoA-binding，ACB）结构域，能与酰基辅酶A酯结合并在真核细胞中运输。虽然ACBP的各种功能已被确定在几种植物物种，其结构，分子进化，表达谱和功能尚未完全阐明inZea maysL.ResultsGenome-wide分析确定了9个ZmACBP基因在Z。通过构建包含6种不同植物48个ACBP基因的系统发育树，将玉米分为4个不同的类群（class I、class II、class III和class IV）。ZmACBP-GFP融合蛋白在烟草表皮细胞中的瞬时表达揭示了ZmACBPs定位于多个不同的位置。表达谱分析表明，ZmACBP抑制非生物和生物胁迫过程中的时空表达变化。在一组500个玉米自交系中，还发现了9个ZmACBP基因中的8个与农艺性状显著相关。ZmACBP 1和ZmACBP 3在拟南芥中的异源组成型表达增强了这些植物对盐和干旱胁迫的抗性，可能是通过改变脂质代谢和胁迫响应基因的水平。结论ACBP基因家族在不同植物中高度保守，ZmACBP基因具有明确的组织和器官表达特异性，对生物和非生物胁迫都有响应，表明它们在植物生长和抗逆中的作用。
BackgroundAcyl-CoA-binding proteins (ACBPs) possess a conserved acyl-CoA-binding (ACB) domain that facilitates binding to acyl-CoA esters and trafficking in eukaryotic cells. Although the various functions of ACBP have been characterized in several plant species, their structure, molecular evolution, expression profile, and function have not been fully elucidated inZea maysL.ResultsGenome-wide analysis identified nineZmACBPgenes inZ. mays, which could be divided into four distinct classes (class I, class II, class III, and class IV) via construction of a phylogenetic tree that included 48ACBPgenes from six different plant species. Transient expression of a ZmACBP-GFP fusion protein in tobacco (Nicotiana tabacum) epidermal cells revealed that ZmACBPs localized to multiple different locations. Analyses of expression profiles revealed thatZmACBPsexhibited temporal and spatial expression changes during abiotic and biotic stresses. Eight of the nineZmACBPgenes were also found to have significant association with agronomic traits in a panel of 500 maize inbred lines. The heterologous constitutive expression ofZmACBP1andZmACBP3in Arabidopsis enhanced the resistance of these plants to salinity and drought stress, possibly through alterations in the level of lipid metabolic and stress-responsive genes.ConclusionTheACBPgene family was highly conserved across different plant species.ZmACBPgenes had clear tissue and organ expression specificity and were responsive to both biotic and abiotic stresses, suggesting their roles in plant growth and stress resistance.
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影响因子：
5.8
作者：
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