Enhancing the oral and topical insecticidal efficacy of a commercialized spider venom peptide biopesticide via fusion to the carrier snowdrop lectin (Galanthus nivalis agglutinin).
Enhancing the oral and topical insecticidal efficacy of a commercialized spider venom peptide biopesticide via fusion to the carrier snowdrop lectin (Galanthus nivalis agglutinin).
复制标题
通过融合到载体雪花蛋白（Galanthus nivalis凝集素）中，增强了商业化蜘蛛肽生物农药的口服和局部杀虫功效。
DOI：
10.1002/ps.7198
复制
发表时间：
2023-01
影响因子：
4.1
通讯作者：
Fitches, Elaine C.
中科院分区：
文献类型：
--
作者：
Sukiran, Nur Afiqah;Pyati, Prashant;Willis, Caitlin E.;Brown, Adrian P.;Readshaw, Jennifer J.;Fitches, Elaine C.
Spear®-T作为接触式叶面喷雾剂销售，用于控制温室害虫，如蚜虫、蓟马、叶螨和粉虱，含有重组蜘蛛毒肽GS-ω/κ-HxTx-Hv 1h（Vestaron命名为GS-ω/κ-HxTx-Hv 1a）作为活性成分。在这里，我们调查是否融合的肽雪花莲凝集素，（雪花莲凝集素; GNA）增强这种毒液肽对蚜虫害虫的功效。重组GS-ω/κ-HxTx-Hv 1h（HxTx-Hv 1h）和HxTx-Hv 1h/GNA融合蛋白使用毕赤酵母生产。纯化的蛋白质注射到鳞翅目（Mamestra brassicae）幼虫时表现出相当的毒性，但对蚜虫的口服和接触活性的显着差异。HxTx-Hv 1h对豌豆（豌豆蚜）和桃马铃薯（桃蚜）蚜虫的急性经口毒性相当，来自饲料测定的第（2）天半数致死浓度（LC 50）值分别为111和108 μm。融合蛋白对两种物种也显示出相当的口服毒性，但与HxTx-Hv 1h相比，D2 LC 50值低3倍以上（豌豆和桃马铃薯蚜虫分别为35和33 μm）。局部应用毒素和融合蛋白，但没有GNA，导致豌豆蚜虫生存显着减少。与单独毒素相比，暴露于融合蛋白的蚜虫对死亡率的接触效应显著更大。全蚜虫荧光显微镜和免疫印迹表明，改善的功效是由于在摄入或局部施用的蛋白质内化后与GNA融合时HxTx-Hv 1h的持久性增强。这是第一项报告HxTx‐ Hv 1h对蚜虫杀虫活性的研究，结果表明，基于融合蛋白的方法提供了显着增强天然衍生毒素（如HxTx‐ Hv 1h）对作物害虫的口服和接触效力的机会。版权所有© 2022作者。由John Wiley & Sons有限公司代表化学工业协会出版的《害虫管理科学》。我们已经产生了重组蜘蛛毒液衍生肽（HxTx-Hv 1h）和将HxTx-Hv 1h连接到雪花莲凝集素（GNA）的融合蛋白，并且随后显示，与HxTx-Hv 1h相比，融合蛋白对两种蚜虫害虫物种具有更大的口服和接触杀虫活性。
Spear®‐T sold as a contact foliar spray for the control of glasshouse pests such as aphids, thrips, spider mites and whiteflies, contains the recombinant spider venom peptide GS‐ω/κ‐HxTx‐Hv1h (named as GS‐ω/κ‐HxTx‐Hv1a by Vestaron) as the active ingredient. Here we investigate whether fusion of the peptide to snowdrop lectin, (Galanthus nivalis agglutinin; GNA) enhances the efficacy of this venom peptide towards aphid pests. Recombinant GS‐ω/κ‐HxTx‐Hv1h (HxTx‐Hv1h) and an HxTx‐Hv1h/GNA fusion protein were produced using the yeast Pichia pastoris. Purified proteins showed comparable toxicity when injected into lepidopteran (Mamestra brassicae) larvae, but significant differences in oral and contact activity towards aphids. HxTx‐Hv1h had comparable acute oral toxicity to pea (Acyrthosiphon pisum) and peach potato (Myzus persicae) aphids with respective Day (2) median lethal concentration (LC50) values of 111 and 108 μm derived from diet assays. The fusion protein also showed comparable oral toxicity to both species but D2 LC50 values were >3‐fold lower (35 and 33 μm for pea and peach potato aphids, respectively) as compared to HxTx‐Hv1h. Topically applied toxin and fusion protein, but not GNA, caused significant reductions in pea aphid survival. Contact effects on mortality were significantly greater for aphids exposed to fusion protein as compared to toxin alone. Whole aphid fluorescence microscopy and immunoblotting suggest that improved efficacy is due to enhanced persistence of HxTx‐Hv1h when fused to GNA following internalisation of ingested or topically applied proteins. This is the first study to report on the insecticidal activity of HxTx‐Hv1h towards aphids and results suggest that a fusion protein‐based approach offers opportunities to significantly enhance oral and contact efficacy of naturally derived toxins, such as HxTx‐Hv1h, towards crop pests. © 2022 The Authors. Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry. We have produced a recombinant spider venom derived peptide (HxTx‐Hv1h) and a fusion protein linking HxTx‐Hv1h to snowdrop lectin (GNA) and subsequently shown that the fusion protein has greater oral and contact insecticidal activity as compared to HxTx‐Hv1h towards two pest species of aphids.
登录
查看更多内容
影响因子：
5.8
作者：
Chong, Youmie;Hayes, Jessica L.;Nicholson, Graham M.
通讯作者：
Nicholson, Graham M.
影响因子：
8
作者：
PALLAGHY, PK;NIELSEN, KJ;NORTON, RS
通讯作者：
NORTON, RS
影响因子：
4.2
作者：
Herzig V;King GF
通讯作者：
King GF
影响因子：
2.8
作者：
Craik, DJ;Daly, NL;Waine, C
通讯作者：
Waine, C
DOI：
10.1098/rspb.2014.0619
发表时间：
2014-07-22
期刊：
Proceedings. Biological sciences
影响因子：
--
作者：
Nakasu EY;Williamson SM;Edwards MG;Fitches EC;Gatehouse JA;Wright GA;Gatehouse AM
通讯作者：
Gatehouse AM