Novel biopesticide based on a spider venom peptide shows no adverse effects on honeybees.
Novel biopesticide based on a spider venom peptide shows no adverse effects on honeybees.
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DOI：
10.1098/rspb.2014.0619
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发表时间：
2014-07-22
期刊：
影响因子：
--
通讯作者：
Gatehouse AM
中科院分区：
文献类型：
--
作者：
Nakasu EY;Williamson SM;Edwards MG;Fitches EC;Gatehouse JA;Wright GA;Gatehouse AM
越来越多的证据表明，常用的杀虫剂与传粉昆虫数量的下降有关;三种类烟碱类化合物对蜜蜂的不利影响已导致整个欧盟禁止使用它们。开发对蜜蜂等有益生物的风险可以忽略不计的杀虫剂是可取和及时的。一种策略是使用含有与赋予口服毒性的“载体”蛋白连接的神经活性肽/蛋白的重组融合蛋白。雪花莲凝集素（Galanthusnivalisagglutinin，Hv 1a/GNA）是一种以雪花莲凝集素（GNA）为载体的昆虫特异性蜘蛛毒钙通道阻断剂（ω-hexatoxin-Hv 1a）。通过全面的早期风险评估，评估了Hv 1a/GNA对非目标物种意大利蜜蜂的影响。喂食后，蜜蜂内化了Hv 1a/GNA，在暴露后1小时内到达大脑。然而，摄取（LD 50> 100 µg bee−1）或注射融合蛋白对存活率只有轻微影响。喂食急性（100 µg bee−1）或慢性（0.35 mg ml−1）剂量的Hv 1a/GNA并接受嗅觉学习任务训练的蜜蜂，其学习和记忆率与无农药对照组相似。幼虫不受影响，能够降解Hv 1a/GNA。这些测试表明，Hv 1a/GNA不太可能对蜜蜂造成有害影响，这表明靶向钙通道的蜜蜂毒素是传统农药的潜在替代品。
Evidence is accumulating that commonly used pesticides are linked to decline of pollinator populations; adverse effects of three neonicotinoids on bees have led to bans on their use across the European Union. Developing insecticides that pose negligible risks to beneficial organisms such as honeybees is desirable and timely. One strategy is to use recombinant fusion proteins containing neuroactive peptides/proteins linked to a ‘carrier’ protein that confers oral toxicity. Hv1a/GNA (Galanthus nivalis agglutinin), containing an insect-specific spider venom calcium channel blocker (ω-hexatoxin-Hv1a) linked to snowdrop lectin (GNA) as a ‘carrier’, is an effective oral biopesticide towards various insect pests. Effects of Hv1a/GNA towards a non-target species, Apis mellifera, were assessed through a thorough early-tier risk assessment. Following feeding, honeybees internalized Hv1a/GNA, which reached the brain within 1 h after exposure. However, survival was only slightly affected by ingestion (LD50 > 100 µg bee−1) or injection of fusion protein. Bees fed acute (100 µg bee−1) or chronic (0.35 mg ml−1) doses of Hv1a/GNA and trained in an olfactory learning task had similar rates of learning and memory to no-pesticide controls. Larvae were unaffected, being able to degrade Hv1a/GNA. These tests suggest that Hv1a/GNA is unlikely to cause detrimental effects on honeybees, indicating that atracotoxins targeting calcium channels are potential alternatives to conventional pesticides.
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影响因子：
6.8
作者：
Decourtye, A;Devillers, J;Pham-Delègue, MH
通讯作者：
Pham-Delègue, MH
影响因子：
6.1
作者：
Jeschke, Peter;Nauen, Ralf;Elbert, Alfred
通讯作者：
Elbert, Alfred
影响因子：
1.4
作者：
BITTERMAN, ME;MENZEL, R;SCHAFER, S
通讯作者：
SCHAFER, S
影响因子：
4.1
作者：
Elbert, Alfred;Haas, Matthias;Nauen, Ralf
通讯作者：
Nauen, Ralf
影响因子：
3.8
作者：
Bravo, Alejandra;Likitvivatanavong, Supaporn;Gill, Sarjeet S.;Soberon, Mario
通讯作者：
Soberon, Mario