Boron deficiency inhibits root cell elongation via an ethylene/auxin/ROS-dependent pathway in Arabidopsis seedlings.
Boron deficiency inhibits root cell elongation via an ethylene/auxin/ROS-dependent pathway in Arabidopsis seedlings.
复制标题
DOI：
10.1093/jxb/erv186
复制
发表时间：
2015-07
影响因子：
6.9
通讯作者：
González-Fontes A
中科院分区：
文献类型：
--
作者：
Camacho-Cristóbal JJ;Martín-Rejano EM;Herrera-Rodríguez MB;Navarro-Gochicoa MT;Rexach J;González-Fontes A
缺硼对根细胞伸长的快速抑制是由乙烯/生长素/ROS依赖的途径介导的。缺硼最早的症状之一是根伸长的抑制，这可以合理地归因于缺硼对细胞壁完整性的破坏作用。结果表明，野生型拟南芥幼苗暴露于缺硼4h后，根细胞长度在伸长区和分化区之间的过渡过程中受到严重的抑制。为了探讨乙烯、生长素和活性氧物种(ROS)在缺硼对根细胞伸长的影响中的可能中介作用，将缺硼与乙烯生物合成的化学抑制剂氨基乙氧基乙烯甘氨酸、银离子(乙烯感受拮抗剂Ag+)、α-(苯乙基-2-氧)吲哚乙酸(合成的TIR1受体功能拮抗剂)和ROS产生的抑制剂二苯基碘(DPI)一起应用。有趣的是，所有这些化学物质都部分或完全恢复了缺硼根的细胞伸长。为了进一步探讨乙烯和生长素在缺硼条件下抑制根细胞伸长中的可能作用，利用乙烯(ein2-1)或生长素(eir1-4和aux 1-22)反应缺陷的拟南芥突变体进行了遗传分析。与野生型相比，这些突变体的根细胞伸长对缺硼处理的敏感性较低。综上所述，这些结果表明，一条涉及乙烯、生长素和ROS的信号通路参与了拟南芥幼苗缺硼时根细胞伸长的减少。在不同类型的细胞壁胁迫下，已经描述了一个类似的信号传递过程来迅速减少根的伸长，这支持了这种信号传递途径是由缺硼导致的细胞壁完整性受损触发的观点。
The rapid inhibition of root cell elongation in response to boron deficiency is mediated by an ethylene/auxin/ROS dependent pathway One of the earliest symptoms of boron (B) deficiency is the inhibition of root elongation which can reasonably be attributed to the damaging effects of B deprivation on cell wall integrity. It is shown here that exposure of wild-type Arabidopsis thaliana seedlings to B deficiency for 4h led to a drastic inhibition of root cell length in the transition between the elongation and differentiation zones. To investigate the possible mediation of ethylene, auxin, and reactive oxygen species (ROS) in the effect of B deficiency on root cell elongation, B deficiency was applied together with aminoethoxyvinylglycine (AVG, a chemical inhibitor of ethylene biosynthesis), silver ions (Ag+, an antagonist of ethylene perception), α-(phenylethyl-2‐oxo)‐indoleacetic acid (PEO-IAA, a synthetic antagonist of TIR1 receptor function), and diphenylene iodonium (DPI, an inhibitor of ROS production). Interestingly, all these chemicals partially or fully restored cell elongation in B-deficient roots. To further explore the possible role of ethylene and auxin in the inhibition of root cell elongation under B deficiency, a genetic approach was performed by using Arabidopsis mutants defective in the ethylene (ein2‐1) or auxin (eir1-4 and aux1-22) response. Root cell elongation in these mutants was less sensitive to B-deficient treatment than that in wild-type plants. Altogether, these results demonstrated that a signalling pathway involving ethylene, auxin, and ROS participates in the reduction of root cell elongation when Arabidopsis seedlings are subjected to B deficiency. A similar signalling process has been described to reduce root elongation rapidly under various types of cell wall stress which supports the idea that this signalling pathway is triggered by the impaired cell wall integrity caused by B deficiency.
登录
查看更多内容
DOI：
10.1111/tpj.12403
发表时间：
2014-02
期刊：
The Plant journal : for cell and molecular biology
影响因子：
--
作者：
Chormova D;Messenger DJ;Fry SC
通讯作者：
Fry SC
影响因子：
7.4
作者：
Kobayashi, M;Matoh, T;Azuma, J
通讯作者：
Azuma, J
影响因子：
5.2
作者：
Beato, Victor M.;Rexach, Jesus;Gonzalez-Fontes, Agustin
通讯作者：
Gonzalez-Fontes, Agustin
影响因子：
5.7
作者：
Camacho-Cristobal, Juan J.;Herrera-Rodriguez, Maria Begona;Gonzalez-Fontes, Agustin
通讯作者：
Gonzalez-Fontes, Agustin
影响因子：
4.9
作者：
Dell, B;Huang, LB
通讯作者：
Huang, LB