Expression of hunchback during trunk segmentation in the branchiopod crustacean Artemia franciscana
Expression of hunchback during trunk segmentation in the branchiopod crustacean Artemia franciscana
复制标题
鳃足类甲壳类丰年虫躯干分割过程中驼背的表达
DOI：
10.1007/s00427-005-0030-y
复制
发表时间：
2006
影响因子：
2.4
通讯作者：
M. Averof
中科院分区：
文献类型：
--
作者：
Zacharias Kontarakis;T. Copf;M. Averof
比较研究表明，在节肢动物中，分割的某些方面是广泛保守的。然而，目前还不清楚是否需要在果蝇分割的分子prepatterns可能是类似的保守在其他节肢动物群体。果蝇间隙基因的同源物，如驼背，在不同昆虫的早期分割阶段显示区域限制的表达模式，但其在其他节肢动物群体中的表达模式尚不清楚。在这里，我们报告的克隆驼背直向同源的甲壳动物卤虫franciscana和它的表达过程中形成的树干段。卤虫hunchback表示在一系列的节段条纹，对应于个人的胸部/躯干，生殖器，和postgenital节。然而，这种表达是不相关的分割外胚层，但限于中胚层细胞，与外胚层在一个规则的同色异谱模式。早期节段中胚层的所有细胞似乎表达hunchback。随后，中胚层表达减弱，并且在中枢神经系统（CNS）中出现复杂的表达模式，这与昆虫CNS中的hunchback表达相当。没有区域限制的表达，让人想起差距基因的表达，观察在树干分割。这些模式表明，hunchback在中胚层和中枢神经系统中的表达模式可能是古老的和保守的甲壳类动物和昆虫。相比之下，我们发现没有证据的保守作用，驼背的轴向图案在躯干外胚层。
Comparative studies have shown that some aspects of segmentation are widely conserved among arthropods. Yet, it is still unclear whether the molecular prepatterns that are required for segmentation in Drosophila are likely to be similarly conserved in other arthropod groups. Homologues of the Drosophila gap genes, like hunchback, show regionally restricted expression patterns during the early phases of segmentation in diverse insects, but their expression patterns in other arthropod groups are not yet known. Here, we report the cloning of a hunchback orthologue from the crustacean Artemia franciscana and its expression during the formation of trunk segments. Artemia hunchback is expressed in a series of segmental stripes that correspond to individual thoracic/trunk, genital, and postgenital segments. However, this expression is not associated with the segmenting ectoderm but is restricted to mesodermal cells that associate with the ectoderm in a regular metameric pattern. All cells in the early segmental mesoderm appear to express hunchback. Later, mesodermal expression fades, and a complex expression pattern appears in the central nervous system (CNS), which is comparable to hunchback expression in the CNS of insects. No regionally restricted expression, reminiscent of gap gene expression, is observed during trunk segmentation. These patterns suggest that the expression patterns of hunchback in the mesoderm and in the CNS are likely to be ancient and conserved among crustaceans and insects. In contrast, we find no evidence for a conserved role of hunchback in axial patterning in the trunk ectoderm.
影响因子：
2.7
作者：
A. H. Werbrock;D. A. Meiklejohn;A. Sainz;J. H. Iwasa;R. M. Savage
通讯作者：
A. H. Werbrock;D. A. Meiklejohn;A. Sainz;J. H. Iwasa;R. M. Savage
驼背直系同源物 Lzf2 的鉴定和表征及其在水蛭胚胎发生过程中的表达。
影响因子：
2.7
作者：
Savage,RM;Shankland,M
通讯作者：
Shankland,M
果蝇分割基因表达的母体控制。
影响因子：
64.8
作者：
Carroll,SB;Winslow,GM;Schupbach,T;Scott,MP
通讯作者：
Scott,MP