Quantitation of tenofovir and emtricitabine in dried blood spots (DBS) with LC-MS/MS.
Quantitation of tenofovir and emtricitabine in dried blood spots (DBS) with LC-MS/MS.
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DOI：
10.1016/j.jpba.2013.08.033
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发表时间：
2014-01
影响因子：
3.4
通讯作者：
Anderson, Peter L.
中科院分区：
文献类型：
--
作者：
Zheng, Jia-Hua;Guida, Louis A.;Rower, Caitlin;Castillo-Mancilla, Jose;Meditz, Amie;Klein, Brandon;Kerr, Becky Jo;Langness, Jacob;Bushman, Lane;Kiser, Jennifer;Anderson, Peter L.
A reversed-phase high performance liquid chromatographic (LC), tandem mass spectrometry (MS/MS) assay for the determination of tenofovir (TFV) and emtricitabine (FTC) in dried blood spots (DBS) from human whole blood was developed and validated. Whole blood samples were spotted, dried, and a 3mm punch was extracted with methanol for analysis by LC-MS/MS utilizing stable isotope labeled internal standards. The assay was validated over the range of 2.5ng/mL to 1,000ng/mL for TFV and 2.5ng/mL to 5,000ng/mL for FTC. The method was accurate (within ± 15% of control) and precise (coefficient of variation ≤ 15%) for hematocrit concentrations ranging from 25% to 76%; using edge punches versus center punches; and spot volumes of 10µL to 50µL. Analytes were stable for five freeze/thaw cycles and up to 6 days at room temperature, whereas long-term storage required −20°C or −80°C. Comparison of TFV and FTC in DBS versus plasma yielded r2 ≥ 0.96, indicating that DBS can be used as a plasma alternative for pharmacokinetic analyses in vivo.
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影响因子：
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