Molecular cloning, phylogenetic analysis, and expression profiling of endoplasmic reticulum molecular chaperone BiP genes from bread wheat (Triticum aestivum L.).
Molecular cloning, phylogenetic analysis, and expression profiling of endoplasmic reticulum molecular chaperone BiP genes from bread wheat (Triticum aestivum L.).
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面包小麦 (Triticum aestivum L.) 内质网分子伴侣 BiP 基因的分子克隆、系统发育分析和表达谱
DOI：
10.1186/s12870-014-0260-0
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发表时间：
2014-10-01
影响因子：
5.3
通讯作者：
Yan Y
中科院分区：
文献类型：
--
作者：
Zhu J;Hao P;Chen G;Han C;Li X;Zeller FJ;Hsam SL;Hu Y;Yan Y
内质网伴侣结合蛋白（BiP）是一种重要的功能蛋白，参与蛋白质的合成、折叠组装和分泌。为了研究BiP在小麦种子发育过程中的作用，我们克隆了3个面包小麦BiP同源cDNA序列（Triticum aestivum）的基因组DNA序列，通过cDNA末端快速扩增（RACE）技术，检测了小麦BiP基因在小麦组织中的表达，特别是利用HMW-GS沉默的近等基因系（NILs）研究了BiP基因表达与HMW-GS亚基类型之间的关系，在非生物胁迫下。序列分析表明，所有BiP均包含植物、动物和微生物中存在的三个高度保守结构域，表明它们在不同生物物种中的进化保守性。定量逆转录-聚合酶链反应（qRT-PCR）显示TaBiP（Triticum aestivum BiP）的表达不是器官特异性的，而是主要定位于种子胚乳。此外，免疫定位证实TaBiP主要定位在小麦胚乳中的蛋白体（PB）中。高分子量麦谷蛋白亚基（HMW-GS）沉默后，3个TaBiP基因的表达显著下调。干旱胁迫诱导小麦根、叶和籽粒中TaBiPs的表达显著上调。BiP序列的高度保守性表明，BiP在新生多肽的折叠和组装以及跨物种的蛋白质合成中发挥相同的作用，或具有共同的机制。 TaBiPs的表达在种子发育和早期幼苗生长过程中以及在各种非生物胁迫下受到发育调节。
The endoplasmic reticulum chaperone binding protein (BiP) is an important functional protein, which is involved in protein synthesis, folding assembly, and secretion. In order to study the role of BiP in the process of wheat seed development, we cloned three BiP homologous cDNA sequences in bread wheat (Triticum aestivum), completed by rapid amplification of cDNA ends (RACE), and examined the expression of wheat BiP in wheat tissues, particularly the relationship between BiP expression and the subunit types of HMW-GS using near-isogenic lines (NILs) of HMW-GS silencing, and under abiotic stress. Sequence analysis demonstrated that all BiPs contained three highly conserved domains present in plants, animals, and microorganisms, indicating their evolutionary conservation among different biological species. Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) revealed that TaBiP (Triticum aestivum BiP) expression was not organ-specific, but was predominantly localized to seed endosperm. Furthermore, immunolocalization confirmed that TaBiP was primarily located within the protein bodies (PBs) in wheat endosperm. Three TaBiP genes exhibited significantly down-regulated expression following high molecular weight-glutenin subunit (HMW-GS) silencing. Drought stress induced significantly up-regulated expression of TaBiPs in wheat roots, leaves, and developing grains. The high conservation of BiP sequences suggests that BiP plays the same role, or has common mechanisms, in the folding and assembly of nascent polypeptides and protein synthesis across species. The expression of TaBiPs is developmentally regulated during seed development and early seedling growth, and under various abiotic stresses.
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DOI：
10.1073/pnas.0408941102
发表时间：
2005-04-05
影响因子：
11.1
作者：
Iwata, Y;Koizumi, N
通讯作者：
Koizumi, N
影响因子：
7.4
作者：
GRIFFITH, M;ALA, P;MOFFATT, BA
通讯作者：
MOFFATT, BA
影响因子：
2.4
作者：
He, ZH;Liu, L;Peña, RJ
通讯作者：
Peña, RJ
影响因子：
1.6
作者：
Iwata, Yuji;Yoneda, Mari;Koizumi, Nozomu
通讯作者：
Koizumi, Nozomu
影响因子：
11.6
作者：
BOSTON, RS;FONTES, EBP;WROBEL, RL
通讯作者：
WROBEL, RL