Coating of biomaterial scaffolds with the collagen-mimetic peptide GFOGER for bone defect repair.
Coating of biomaterial scaffolds with the collagen-mimetic peptide GFOGER for bone defect repair.
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DOI：
10.1016/j.biomaterials.2009.12.008
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发表时间：
2010-03
期刊：
影响因子：
14
通讯作者：
Garcia, Andres J.
中科院分区：
文献类型：
--
作者：
Wojtowicz, Abigail M.;Shekaran, Asha;Oest, Megan E.;Dupont, Kenneth M.;Templeman, Kellie L.;Hutmacher, Dietmar W.;Guldberg, Robert E.;Garcia, Andres J.
修复大型骨缺损和骨不连仍然是一个重要的临床问题。目前的治疗方法包括自体和同种异体移植，受到供体供应和发病率、生物活性不足和感染风险的限制。生物疗法，包括细胞、基因和蛋白质，代表着很有前途的替代疗法，但这些策略受到生物治疗传递的技术障碍、细胞来源、高成本和监管障碍的限制。在本研究中，模拟胶原多肽GFOGER被用来包裹合成的PCL支架，以促进大鼠严重节段性缺损处的骨形成。GFOGER是一种人工合成的三螺旋多肽，与参与成骨作用的α2β1整合素受体结合。在没有外源细胞或生长因子的情况下，GFOGER涂层被动吸附到聚合物支架上，与未涂层支架和空白缺损相比，显著加速和增加了未愈合股骨缺损的骨形成。尽管骨体积有差异，但12周后扭转强度没有差异，表明该模型改善的是骨量，而不是骨质量。这项工作展示了一种简单的、不含细胞/生长因子的策略，以促进具有挑战性的、不可愈合的骨缺损的骨形成。这种生物材料涂层策略代表了一种具有成本效益和简单易行的方法，可转化为肌肉骨骼应用的强大临床治疗。
Healing large bone defects and non-unions remains a significant clinical problem. Current treatments, consisting of auto- and allografts, are limited by donor supply and morbidity, insufficient bioactivity and risk of infection. Biotherapeutics, including cells, genes and proteins, represent promising alternative therapies, but these strategies are limited by technical roadblocks to biotherapeutic delivery, cell sourcing, high cost, and regulatory hurdles. In the present study, the collagen-mimetic peptide, GFOGER, was used to coat synthetic PCL scaffolds to promote bone formation in critically-sized segmental defects in rats. GFOGER is a synthetic triple helical peptide that binds to the α2β1 integrin receptor involved in osteogenesis. GFOGER coatings passively-adsorbed onto polymeric scaffolds, in the absence of exogenous cells or growth factors, significantly accelerated and increased bone formation in non-healing femoral defects compared to uncoated scaffolds and empty defects. Despite differences in bone volume, no differences in torsional strength were detected after 12 weeks, indicating that bone mass but not bone quality was improved in this model. This work demonstrates a simple, cell/growth factor-free strategy to promote bone formation in challenging, non-healing bone defects. This biomaterial coating strategy represents a cost effective and facile approach translatable into a robust clinical therapy for musculoskeletal applications.
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影响因子：
14
作者：
García, AJ
通讯作者：
García, AJ
DOI：
10.1097/01.blo.0000150371.77314.52
发表时间：
2005-03-01
影响因子：
4.2
作者：
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通讯作者：
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影响因子：
14
作者：
Hennessy, Kristin M.;Pollot, Beth E.;Clem, William C.;Phipps, Matthew C.;Sawyer, Amber A.;Culpepper, Bonnie K.;Bellis, Susan L.
通讯作者：
Bellis, Susan L.
影响因子：
6.2
作者：
Jikko, A;Harris, SE;Damsky, CH
通讯作者：
Damsky, CH