The effect of collagen I mimetic peptides on mesenchymal stem cell adhesion and differentiation, and on bone formation at hydroxyapatite surfaces.
The effect of collagen I mimetic peptides on mesenchymal stem cell adhesion and differentiation, and on bone formation at hydroxyapatite surfaces.
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DOI：
10.1016/j.biomaterials.2008.12.053
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发表时间：
2009-04
期刊：
影响因子：
14
通讯作者：
Bellis, Susan L.
中科院分区：
文献类型：
--
作者：
Hennessy, Kristin M.;Pollot, Beth E.;Clem, William C.;Phipps, Matthew C.;Sawyer, Amber A.;Culpepper, Bonnie K.;Bellis, Susan L.
整合素结合肽可增加细胞对天然羟基磷灰石 (HA) 的粘附，但在体内，HA 会因蛋白质吸附而迅速发生修饰。之前我们报道过，当与吸附的蛋白质层结合时，RGD 肽会干扰细胞与 HA 的粘附。在当前的研究中，我们评估了间充质干细胞 (MSC) 与涂有模拟胶原肽、DGEA、P15 和 GFOGER 的 HA 盘的相互作用。 MSC 与涂有 DGEA、P15 或 I 型胶原蛋白的盘的粘附效果相同，所有三种基质（但 GFOGER 除外）比未涂层的 HA 都支持更好的细胞粘附。当肽包被的盘上涂有来自血清或胫骨微环境的蛋白质时，胶原模拟物不会抑制 MSC 粘附，正如用 RGD 观察到的那样，但它们也不会增强粘附。鉴于胶原选择性整合素的激活会刺激成骨细胞分化，我们监测了粘附在 DGEA 或 P15 涂层盘上的 MSC 的骨钙素分泌和碱性磷酸酶活性。在存在和不存在分化诱导培养基的情况下，这两种成骨细胞标记物均被 DGEA 和 P15 上调。最后，胶原模拟物增加了 HA 胫骨植入物上的骨形成。总的来说，这些结果表明，胶原模拟肽改善 HA 的骨整合，最有可能是通过刺激 MSC 的成骨细胞分化，而不是粘附。
Integrin-binding peptides increase cell adhesion to naive hydroxyapatite (HA), however, in the body, HA becomes rapidly modified by protein adsorption. Previously we reported that, when combined with an adsorbed protein layer, RGD peptides interfered with cell adhesion to HA. In the current study we evaluated mesenchymal stem cell (MSC) interactions with HA disks coated with the collagen-mimetic peptides, DGEA, P15 and GFOGER. MSCs adhered equally well to disks coated with DGEA, P15, or collagen I, and all three substrates, but not GFOGER, supported greater cell adhesion than uncoated HA. When peptide-coated disks were overcoated with proteins from serum or the tibial microenvironment, collagen mimetics did not inhibit MSC adhesion, as was observed with RGD, however neither did they enhance adhesion. Given that activation of collagen-selective integrins stimulates osteoblastic differentiation, we monitored osteocalcin secretion and alkaline phosphatase activity from MSCs adherent to DGEA or P15-coated disks. Both of these osteoblastic markers were upregulated by DGEA and P15, in the presence and absence of differentiation-inducing media. Finally, bone formation on HA tibial implants was increased by the collagen-mimetics. Collectively these results suggest that collagen-mimetic peptides improve osseointegration of HA, most probably by stimulating osteoblastic differentiation, rather than adhesion, of MSCs.
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DOI：
10.1002/jbm.b.30541
发表时间：
2006-11-01
影响因子：
3.4
作者：
Leupold, John A.;Barfield, William R.;Hartsock, Langdon A.
通讯作者：
Hartsock, Langdon A.
影响因子：
4.3
作者：
Barros, Raquel R. M.;Novaes, Arthur B., Jr.;Palioto, Daniela B.
通讯作者：
Palioto, Daniela B.
影响因子：
--
作者：
Bhatnagar, RS;Qian, JJ;Smith, N
通讯作者：
Smith, N
影响因子：
4.9
作者：
Kilpadi, KL;Sawyer, AA;Bellis, SL
通讯作者：
Bellis, SL
影响因子：
4.9
作者：
Behravesh, E;Mikos, AG
通讯作者：
Mikos, AG