The role of glucose metabolism in a pig heart model of short-term hibernation
The role of glucose metabolism in a pig heart model of short-term hibernation
复制标题
葡萄糖代谢在猪心脏短期冬眠模型中的作用
DOI：
10.1023/a:1006839022943
复制
发表时间：
1998
影响因子：
4.3
通讯作者：
A. Liedtke
中科院分区：
文献类型：
--
作者：
Timothy A. Hacker;B. Renstrøm;S. Nellis;A. Liedtke
此前，我们报道过，慢性冠状动脉狭窄 4 天模型中葡萄糖代谢的变化与冬眠心脏患者中描述的相似。这项研究的目的有两个：(1) 确定轻度、持续缺血的急性模型是否会产生类似的变化，(2) 确定糖酵解的药理抑制作用。在第一组中，体外灌注的完整猪心脏的左前降支 (LAD) 冠状动脉血流在 85 分钟内减少 40%。第二组接受相同的方案，不同之处在于 LAD 局部缺血 40 分钟后，施用碘乙酸 (IAA) 来阻断糖酵解。缺血使两组的 MVO2 均降低 10%，IAA 治疗后进一步降低 20%。局部收缩期缩短因缺血而减少了近 50%，并且在 IAA 治疗后又减少了 40%。第一组中，由于缺血，糖酵解增加了 700% 以上。与之前的缺血期相比，IAA 导致糖酵解减少 3 倍，并抑制乳酸产生。第一组中的脂肪酸代谢因缺血而显着减少，但 IAA 组中的脂肪酸代谢并未减少。与肌原纤维相关的肌酸激酶活性降低，可能导致收缩功能障碍。总之，这种短期冬眠的急性模型展示了先前报道的慢性冬眠中的几种代谢变化，并且可能有助于确定模拟慢性冠状动脉狭窄和冬眠条件下的底物利用机制。
Previously, we reported, alterations in glucose metabolism in a 4 day model of chronic coronary stenosis similar to those described in patients with hibernating hearts. The purpose of this study was 2 fold: (1) to identify whether an acute model of mild, sustained ischemia could effect similar changes, and (2) to determine the effects of pharmacological inhibition of glycolysis. In the first group, extracorporeally perfused, intact pig hearts were subjected to 85 min of a 40% reduction in left anterior descending (LAD) coronary arterial blood flow. A second group was subjected to the same protocol, except after 40 min of LAD regional ischemia, iodoacetate (IAA) was administered to block glycolysis. Ischemia reduced MVO2 by 10% in both groups with a further 20% reduction noted following IAA treatment. Regional systolic shortening was reduced nearly 50% by ischemia and decreased an additional 40% following treatment with IAA. Glycolysis was increased by over 700% with ischemia in the first group. IAA caused a 3 fold reduction in glycolysis as compared to the preceding ischemic period and inhibited lactate production. Fatty acid metabolism was significantly reduced by ischemia in the first group, but was not reduced in the IAA group. Activity of creatine kinase associated with myofibrils was reduced and may have contributed to the contractile dysfunction. In conclusion, this acute model of short-term hibernation demonstrates several metabolic changes previously reported in chronic hibernation and may prove useful in determining mechanisms of substrate utilization in simulated conditions of chronic coronary stenosis and hibernation.
登录
查看更多内容
完全缺血III：无氧糖酵解的抑制作用。
影响因子：
5
作者：
Jennings,RB;Reimer,KA;SteenbergenJr,C;Schaper,J
通讯作者：
Schaper,J
大鼠冠状动脉非闭塞性收缩引起的非缺血性心肌损伤。
DOI：
10.1152/ajpheart.1991.260.3.h651
发表时间：
1991
期刊：
The American journal of physiology
影响因子：
--
作者：
Capasso,JM;Li,P;Anversa,P
通讯作者：
Anversa,P
慢性部分冠状动脉狭窄缓解后猪心脏的心肌功能和代谢。
DOI：
10.1152/ajpheart.1994.267.4.h1312
发表时间：
1994
期刊：
The American journal of physiology
影响因子：
--
作者：
Liedtke,AJ;Renstrom,B;Nellis,SH;Subramanian,R
通讯作者：
Subramanian,R
缺血和再灌注心肌中脂肪酸氧化的改变。
影响因子：
4.3
作者：
Huang,XQ;Liedtke,AJ
通讯作者：
Liedtke,AJ
影响因子：
20.1
作者：
PANTELY, GA;MALONE, SA;BRISTOW, JD
通讯作者：
BRISTOW, JD