Passive skeletal muscle can function as an osmotic engine
Passive skeletal muscle can function as an osmotic engine
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被动骨骼肌可以充当渗透引擎
DOI：
10.1098/rsbl.2020.0738
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发表时间：
2021
期刊：
影响因子：
3.3
通讯作者：
Roberts, Thomas J.
中科院分区：
文献类型：
--
作者：
Wold, Ethan S.;Sleboda, David A.;Roberts, Thomas J.
肌肉是复合结构。负责产生力的蛋白质细丝被捆绑在充满液体的细胞内，这些细胞被包裹在有序的纤维胶原蛋白套筒中。最近的模型表明，细胞内液和细胞外胶原之间的机械相互作用是必不可少的被动骨骼肌中的力的产生，允许材料刚度的细胞外胶原，以促进被动肌肉力在生理相关的肌肉长度。这样的模型导致预测，在这里测试，肌肉内的液体室的扩张应该驱动有力的肌肉缩短，从而产生与收缩活动无关的机械功。我们通过实验增加液体体积的孤立牛蛙半膜肌肌肉，通过approstically低渗洗浴液来测试这一预测。随着时间的推移，浸泡在低渗溶液中的被动肌增宽了16.44 ± 3.66%（平均值± s.d.）。因为它们吸收了液体。与此同时，肌肉沿着其作用线缩短了2.13 ± 0.75%，取代了力调节的肌肉，并做了可测量的机械功。这种行为与对各向同性生物组织的期望相矛盾，该生物组织在内部加压时会延长，这表明了类似于工程气动致动器的功能机制，并突出了骨骼肌中三维力传递的重要性。
Muscles are composite structures. The protein filaments responsible for force production are bundled within fluid-filled cells, and these cells are wrapped in ordered sleeves of fibrous collagen. Recent models suggest that the mechanical interaction between the intracellular fluid and extracellular collagen is essential to force production in passive skeletal muscle, allowing the material stiffness of extracellular collagen to contribute to passive muscle force at physiologically relevant muscle lengths. Such models lead to the prediction, tested here, that expansion of the fluid compartment within muscles should drive forceful muscle shortening, resulting in the production of mechanical work unassociated with contractile activity. We tested this prediction by experimentally increasing the fluid volumes of isolated bullfrog semimembranosus muscles via osmotically hypotonic bathing solutions. Over time, passive muscles bathed in hypotonic solution widened by 16.44 ± 3.66% (mean ± s.d.) as they took on fluid. Concurrently, muscles shortened by 2.13 ± 0.75% along their line of action, displacing a force-regulated servomotor and doing measurable mechanical work. This behaviour contradicts the expectation for an isotropic biological tissue that would lengthen when internally pressurized, suggesting a functional mechanism analogous to that of engineered pneumatic actuators and highlighting the significance of three-dimensional force transmission in skeletal muscle.
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影响因子：
2.4
作者：
Stutzig, Norman;Ryan, David;Siebert, Tobias
通讯作者：
Siebert, Tobias
肌肉力量取决于横向肌肉负荷的大小
影响因子：
2.4
作者：
T. Siebert;O. Till;N. Stutzig;M. Günther;R. Blickhan
通讯作者：
R. Blickhan
DOI：
10.1073/pnas.1914433117
发表时间：
2019
期刊：
Proceedings of the National Academy of Sciences
影响因子：
--
作者：
Sleboda, David A.;Roberts, Thomas J.
通讯作者：
Roberts, Thomas J.
DOI：
10.1242/jeb.209668
发表时间：
2019
期刊：
The Journal of Experimental Biology
影响因子：
--
作者：
Sleboda, David A.;Wold, Ethan S.;Roberts, Thomas J.
通讯作者：
Roberts, Thomas J.
将运动神经元活动映射到水蛭的明显行为
DOI：
--
发表时间：
1996
期刊：
Journal of Comparative Physiology
影响因子：
--
作者：
R. J. Wilson;B. A. Skierczynski;J. Meyer;R. Skalak;W. Kristan
通讯作者：
W. Kristan