Cytochromes P450 2C1/2 and P450 2E1 are retained in the endoplasmic reticulum membrane by different mechanisms.
Cytochromes P450 2C1/2 and P450 2E1 are retained in the endoplasmic reticulum membrane by different mechanisms.
复制标题
细胞色素 P450 2C1/2 和 P450 2E1 通过不同的机制保留在内质网膜中。
DOI：
10.1006/abbi.1999.1628
复制
发表时间：
2000
影响因子：
3.9
通讯作者：
Kemper,B
中科院分区：
文献类型：
--
作者：
Szczesna-Skorupa,E;Chen,CD;Kemper,B
细胞色素 P450 (P450) 2C1/2 含有多余的内质网 (ER) 保留信号，被排除在回收途径之外。其他 P450，例如 P450 2E1，已在质膜和高尔基体中检测到。为了检查 P450 2C1/2 和 P450 2E1 的 ER 保留机制是否可能不同，绿色荧光蛋白和全长蛋白、N 端信号/锚定序列或这些蛋白的细胞质催化结构域的嵌合体已在 COS1 细胞中表达。具有 N 端信号/锚定序列或 P450 2C1/2 胞质结构域的嵌合体保留在 ER 中，并且诺考达唑处理不会改变其分布。具有全长 P450 2E1 的嵌合体位于 ER 中，但与 P450 2C1/2 相比，诺考达唑处理导致重新分布为囊泡模式，这表明该蛋白质通过修复机制保留在 ER 中。为了支持这种可能性，P450 2E1 嵌合体（而不是 P450 2C1/2 嵌合体）被包含在体外出芽测定中产生的转运囊泡中。仅具有与绿色荧光蛋白融合的 P450 2E1 的 N 端信号/锚定序列的嵌合体位于内质网中，诺考达唑处理改变了其分布，而仅具有 P450 2E1 胞质结构域的嵌合体不能有效地保留在内质网中，并且主要积累在高尔基体区域中。这些结果表明，P450 超家族的两个密切相关的成员在 ER 中的保留机制是不同的，并且 N 端信号/锚定序列包含显性保留信号。
Cytochrome P450 (P450) 2C1/2 contains redundant endoplasmic reticulum (ER) retention signals and is excluded from the recycling pathway. Other P450s, such as P450 2E1, have been detected in the plasma membrane and Golgi apparatus. To examine whether the mechanisms of ER retention might differ for P450 2C1/2 and P450 2E1, chimeras of green flourescent protein and the full-length proteins, N-terminal signal/anchor sequences, or the cytoplasmic catalytic domains from these proteins have been expressed in COS1 cells. Chimeras with either the N-terminal signal/anchor sequence or the cytoplasmic domain of P450 2C1/2 were retained in the ER and the distribution was not altered by treatment with nocodazole. A chimera with full-length P450 2E1 was located in the ER, but in contrast to P450 2C1/2, treatment with nocodazole resulted in redistribution to a vesicular pattern, which suggested that this protein was retained in the ER by a retrieval mechanism. In support of this possibility, the P450 2E1 chimera, but not the P450 2C1/2 chimera, was included in transport vesicles generated in an in vitro budding assay. A chimera with only the N-terminal signal/anchor sequence of P450 2E1 fused to green fluorescent protein was located in the ER and nocodazole treatment altered its distribution, whereas a chimera with only the cytoplasmic domain of P450 2E1 was not efficiently retained in the ER and accumulated primarily in the Golgi region. These results demonstrate that the mechanisms for retention in the ER of two closely related members of the P450 superfamily are different and that the N-terminal signal/anchor sequence contains the dominant retention signal.
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影响因子：
2.9
作者：
M. Umeno;O. McBride;Chung S. Yang;H. Gelboin;F. J. Gonzalez
通讯作者：
M. Umeno;O. McBride;Chung S. Yang;H. Gelboin;F. J. Gonzalez
影响因子：
3.9
作者：
C. Yun;T. Ahn;F. Guengerich;H. Yamazaki;T. Shimada
通讯作者：
C. Yun;T. Ahn;F. Guengerich;H. Yamazaki;T. Shimada
内质网和反式高尔基体网络之间多个囊泡转运步骤的差异抑制。
DOI：
--
发表时间：
1993
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Davidson,HW;Balch,WE
通讯作者：
Balch,WE
影响因子：
11.4
作者：
JACKSON, MR;NILSSON, T;PETERSON, PA
通讯作者：
PETERSON, PA
影响因子：
56.9
作者：
Nishimura, N;Balch, WE
通讯作者：
Balch, WE