Trypanosoma cruzi invasion is associated with trogocytosis.
Trypanosoma cruzi invasion is associated with trogocytosis.
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DOI：
10.1016/j.micinf.2014.10.009
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发表时间：
2015-01
影响因子：
5.8
通讯作者：
Tanowitz HB
中科院分区：
文献类型：
--
作者：
Mukherjee S;Mukhopadhyay A;Andriani G;Machado FS;Ashton AW;Huang H;Weiss LM;Tanowitz HB
最初认为胞啃症仅限于免疫系统细胞的相互作用以及这些细胞与癌细胞的相互作用。这种膜交换可能是细胞生物学中的一般过程，并且已经证明膜交换发生在生物体内的非免疫细胞之间。在此，我们报告，膜和蛋白质交换，符合胞刺，克氏锥虫（巴西和Tulahuen株）和哺乳动物细胞感染。通过标记寄生虫或宿主细胞（即人包皮成纤维细胞和大鼠成肌细胞）监测标记的膜贴片的转移。锥鞭毛体和无鞭毛体可将特异性表面糖蛋白随细胞膜沿着转移到宿主细胞中。寄生虫和宿主细胞之间的膜交换发生在成功的入侵。细胞外无鞭毛体不转移膜补丁和热杀死的锥鞭毛体不转移膜或蛋白质到宿主细胞。膜交换也被发现发生在无细胞培养中相互作用的外鞭毛体之间，并且在寄生虫-寄生虫相互作用中也可能是重要的。进一步的研究应提供新的见解发病机制，并提供治疗干预的目标。
Trogocytosis was originally thought to be restricted to the interaction of cells of the immune system and interactions of these cells with cancer cells. Such membrane exchanges are probably a general process in cell biology, and membrane exchange has been demonstrated to occur between non-immune cells within an organism. Herein, we report that membrane and protein exchange, consistent with trogocytosis, between Trypanosoma cruzi (both the Brazil and Tulahuen strains) and the mammalian cells it infects. Transfer of labeled membrane patches was monitored by labeling of either parasites or host cells, i.e. human foreskin fibroblasts and rat myoblasts. Trypomastigotes and amastigotes transferred specific surface glycoproteins to the host cells along with membranes. Exchange of membranes between the parasite and host cells occurred during successful invasion. Extracellular amastigotes did not transfer membrane patches and heat killed trypomastigotes were did not transfer either membranes or proteins to the host cells. Membrane exchange was also found to occur between interacting epimastigotes in cell-free culture and may be important in parasite-parasite interactions as well. Further studies should provide new insights into pathogenesis and provide targets for therapeutic intervention.
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影响因子：
64.8
作者：
通讯作者：
--
影响因子：
21.3
作者：
Marston, DJ;Dickinson, S;Nobes, CD
通讯作者：
Nobes, CD
IgM抗体在H-2激活的小鼠T淋巴细胞上的特异性吸附。
影响因子：
15.3
作者：
Hudson, L;Sprent, J
通讯作者：
Sprent, J
影响因子：
3.8
作者：
Hidron AI;Gilman RH;Justiniano J;Blackstock AJ;Lafuente C;Selum W;Calderon M;Verastegui M;Ferrufino L;Valencia E;Tornheim JA;O'Neal S;Comer R;Galdos-Cardenas G;Bern C;Chagas Disease Working Group in Peru and Bolivia
通讯作者：
Chagas Disease Working Group in Peru and Bolivia
影响因子：
4.4
作者：
Onfelt, Born;Nedvetzki, Shlomo;Davis, Daniel M.
通讯作者：
Davis, Daniel M.