A Sensitive and Validated Method for Determination of T-2 and HT-2 Toxin Residues in Shrimp Tissues by LC-MS/MS
A Sensitive and Validated Method for Determination of T-2 and HT-2 Toxin Residues in Shrimp Tissues by LC-MS/MS
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采用 LC-MS/MS 测定虾组织中 T-2 和 HT-2 毒素残留的灵敏且经过验证的方法
DOI：
10.1007/s12161-015-0336-y
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发表时间：
2016-06
影响因子：
2.9
通讯作者：
Gooneratne Ravi
中科院分区：
文献类型：
--
作者：
Lu Pengli;Wu Chaojin;Shi Qi;Wang Yaling;Sun Lijun;Liao Jianmeng;Zhong Saiyi;Xu Defeng;Chen Jinjun;Liu Ying;Li Jianrong;Gooneratne Ravi
Accurate analyses of T-2 and HT-2 toxin in aquatic organisms including shrimp are important as these two toxins are increasingly detected in aquatic cereal-based feed. Therefore, the potential for these toxins to enter the human food chain from contaminated fish and shrimp products is very real. A rapid, sensitive, and validated method for simultaneous determination of T-2 and HT-2 toxins was developed using a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method following extraction of the two toxins from shrimp tissues with ethyl acetate. This method is simple in that additional solid-phase extraction is not required to isolate and purify the toxins. LC was performed on an analytical Hypersil GOLD column. The mobile phase consisted of methanol and 5 mM ammonium acetate containing 0.1 % formic acid. The MS/MS ion transitions for both the T-2 toxin (484.20 → 214.87) and HT-2 toxin (442.20 → 214.96) were monitored. And the most intense transition ion product (m/z) of T-2 and HT-2 used for quantification on the SRM mode of a mass spectrometer was 304.95 and 262.91, respectively. The results linearly correlated with coefficients >0.9990. The limits of quantification ranged from 0.02 to 0.51 ng·g−1and from 0.17 to 4.48 ng·g−1for T-2 and HT-2, respectively, depending on the shrimp tissue type. The overall extraction recovery for both toxins ranged between 84 and 111 % with RSD values less than 15.0 %, indicative of good replication. Furthermore, the recovery and precision levels were within the predefined limits (≤15 %) at all concentrations. The application of this method to study the accumulation of T-2 toxin in shrimp showed that it can be successfully used to monitor even very low tissue toxin concentrations. Research is in progress to extend this method for the measurement of T-2 and HT-2 in aquatic foods that enter the human food chain.
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DOI：
--
发表时间：
2002
期刊：
World Health Organization technical report series
影响因子：
--
作者：
通讯作者：
--
影响因子：
1.6
作者：
Senyuva, Hamide Z.;Gilbert, John;Donnelly, Carol
通讯作者：
Donnelly, Carol
DOI：
10.1016/s0021-9673(99)00890-0
发表时间：
2000-02
期刊：
Journal of chromatography. A
影响因子：
--
作者：
Misericordia Jiménez;J. J. Mateo-J.;R. Mateo
通讯作者：
Misericordia Jiménez;J. J. Mateo-J.;R. Mateo
影响因子：
3.8
作者：
W. Thompson;Robert W. Wannemacher
通讯作者：
W. Thompson;Robert W. Wannemacher
DOI：
--
发表时间：
2009
期刊：
Journal of Instrumental Analysis
影响因子：
--
作者：
Chen Mingxue
通讯作者：
Chen Mingxue