Metabolic engineering of hydroxy fatty acid production in plants: RcDGAT2 drives dramatic increases in ricinoleate levels in seed oil.
Metabolic engineering of hydroxy fatty acid production in plants: RcDGAT2 drives dramatic increases in ricinoleate levels in seed oil.
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DOI：
10.1111/j.1467-7652.2008.00361.x
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发表时间：
2008-10
影响因子：
13.8
通讯作者：
Browse J
中科院分区：
文献类型：
--
作者：
Burgal J;Shockey J;Lu C;Dyer J;Larson T;Graham I;Browse J
绿色化学的一个中心目标是在油籽作物中生产工业上有用的脂肪酸。虽然从许多野生物种中可以获得编码合适脂肪酸修饰酶的基因，但由于这些基因在转基因植物中的表达产生所需产品的产量很低，因此进展有限。例如，蓖麻（Ricinus communis）脂肪酸羟化酶12 （FAH12）在拟南芥中表达时，最多只能产生17%的羟基脂肪酸（hfa）。鉴定了用于种子油生物合成途径附加步骤的红豆酶的cDNA克隆。这些cdna在FAH12转基因植株中的表达表明，红豆2型酰基辅酶A：二酰基甘油酰基转移酶（RcDGAT2）可使hfa含量从17%提高到近30%。对单转基因和双转基因品系种子中性脂的详细比较表明，RcDGAT2显著修饰了三酰基甘油（TAG）库，在天然蓖麻豆油中观察到的大多数主要TAG物种显著增加。这些数据表明，RcDGAT2倾向于酰基辅酶A和含有hfa的二酰基甘油底物，酵母细胞中表达的RcDGAT2的生化分析证实了它对含有hfa的二酰基甘油底物的强烈偏好。我们的研究结果表明，途径工程方法可以成功地用于提高植物工业原料的产量，并且DGAT2基因家族的成员可能在这一过程中发挥关键作用。
A central goal of green chemistry is to produce industrially useful fatty acids in oilseed crops. Although genes encoding suitable fatty acid-modifying enzymes are available from many wild species, progress has been limited because the expression of these genes in transgenic plants produces low yields of the desired products. For example, Ricinus communis fatty acid hydroxylase 12 (FAH12) produces a maximum of only 17% hydroxy fatty acids (HFAs) when expressed in Arabidopsis. cDNA clones encoding R. communis enzymes for additional steps in the seed oil biosynthetic pathway were identified. Expression of these cDNAs in FAH12 transgenic plants revealed that the R. communis type-2 acyl-coenzyme A:diacylglycerol acyltransferase (RcDGAT2) could increase HFAs from 17% to nearly 30%. Detailed comparisons of seed neutral lipids from the single- and double-transgenic lines indicated that RcDGAT2 substantially modified the triacylglycerol (TAG) pool, with significant increases in most of the major TAG species observed in native castor bean oil. These data suggest that RcDGAT2 prefers acyl-coenzyme A and diacylglycerol substrates containing HFAs, and biochemical analyses of RcDGAT2 expressed in yeast cells confirmed a strong preference for HFA-containing diacylglycerol substrates. Our results demonstrate that pathway engineering approaches can be used successfully to increase the yields of industrial feedstocks in plants, and that members of the DGAT2 gene family probably play a key role in this process.
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影响因子：
7.4
作者：
Knutzon, DS;Hayes, TR;Voelker, TA
通讯作者：
Voelker, TA
影响因子：
7.2
作者：
Clough, SJ;Bent, AF
通讯作者：
Bent, AF
影响因子：
4.1
作者：
BAFOR, M;SMITH, MA;STYMNE, S
通讯作者：
STYMNE, S
影响因子：
11.6
作者：
DEWEY, RE;WILSON, RF;GOODE, JH
通讯作者：
GOODE, JH
DOI：
10.1073/pnas.120067297
发表时间：
2000-06-06
影响因子：
11.1
作者：
Dahlqvist, A;St책hl, U;Stymne, H
通讯作者：
Stymne, H