Phosphorylated nucleolin interacts with translationally controlled tumor protein during mitosis and with Oct4 during interphase in ES cells.
Phosphorylated nucleolin interacts with translationally controlled tumor protein during mitosis and with Oct4 during interphase in ES cells.
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DOI：
10.1371/journal.pone.0013678
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发表时间：
2010-10-27
期刊：
影响因子：
3.7
通讯作者：
Simonsson S
中科院分区：
文献类型：
--
作者：
Johansson H;Svensson F;Runnberg R;Simonsson T;Simonsson S
通过体细胞核移植(SCNT)或诱导多能干细胞(IPS)程序对体细胞进行重新编程以获得胚胎干细胞(ES细胞)或类似ES细胞，为非免疫原性细胞替代治疗提供了潜在的途径。核仁蛋白是胚胎基因激活的标志，其表达对成功的再编程至关重要。虽然核仁蛋白(NCL)是最丰富的核仁蛋白之一，但其在ES细胞中的相互作用伙伴尚不清楚。在这里，我们利用原位邻近连接实验、共定位和免疫沉淀(IP)在ES细胞中探索了新的NCL相互作用蛋白。我们发现在小鼠ES细胞中，磷酸化的NCL(NCL-P)与翻译控制的肿瘤蛋白(Tpt1)相互作用。NCL-P/Tpt1复合体在有丝分裂过程中达到高峰，并在维甲酸诱导分化时减少，这表明它在细胞增殖中发挥作用。此外，我们还发现NCL-P在人和小鼠ES细胞中与转录因子Oct4在间期相互作用，表明NCL-P在转录中起作用。NCL-P/Oct4复合体在人类ES细胞自发分化的早期达到高峰，可能参与了哺乳动物发育的初始分化事件(S)。在这里，我们描述了ES细胞中两种新的蛋白质-蛋白质相互作用，这让我们进一步了解多能细胞中相互作用蛋白质的复杂网络。
Reprogramming of somatic cells for derivation of either embryonic stem (ES) cells, by somatic cell nuclear transfer (SCNT), or ES-like cells, by induced pluripotent stem (iPS) cell procedure, provides potential routes toward non-immunogenic cell replacement therapies. Nucleolar proteins serve as markers for activation of embryonic genes, whose expression is crucial for successful reprogramming. Although Nucleolin (Ncl) is one of the most abundant nucleolar proteins, its interaction partners in ES cells have remained unidentified. Here we explored novel Ncl-interacting proteins using in situ proximity ligation assay (PLA), colocalization and immunoprecipitation (IP) in ES cells. We found that phosphorylated Ncl (Ncl-P) interacted with translationally controlled tumor protein (Tpt1) in murine ES cells. The Ncl-P/Tpt1 complex peaked during mitosis and was reduced upon retinoic acid induced differentiation, signifying a role in cell proliferation. In addition, we showed that Ncl-P interacted with the transcription factor Oct4 during interphase in human as well as murine ES cells, indicating of a role in transcription. The Ncl-P/Oct4 complex peaked during early stages of spontaneous human ES cell differentiation and may thus be involved in the initial differentiation event(s) of mammalian development. Here we described two novel protein-protein interactions in ES cells, which give us further insight into the complex network of interacting proteins in pluripotent cells.
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影响因子：
9.7
作者：
Liu, HT;Yung, BYM
通讯作者：
Yung, BYM
影响因子：
14.9
作者：
Greasley, PJ;Bonnard, C;Amati, B
通讯作者：
Amati, B
影响因子：
4.8
作者：
Grinstein, Edgar;Shan, Ying;Wernet, Peter
通讯作者：
Wernet, Peter
DOI：
10.1073/pnas.94.8.3605
发表时间：
1997-04-15
影响因子：
11.1
作者：
Hanakahi, LA;Dempsey, LA;Maizels, N
通讯作者：
Maizels, N
影响因子：
4.7
作者：
Bazile, Franck;Pascal, Aude;Kubiak, Jacek Z.
通讯作者：
Kubiak, Jacek Z.