A divergent Tbx6-related gene and Tbx6 are both required for neural crest and intermediate mesoderm development in Xenopus.
A divergent Tbx6-related gene and Tbx6 are both required for neural crest and intermediate mesoderm development in Xenopus.
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DOI：
10.1016/j.ydbio.2010.01.013
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发表时间：
2010-04-01
影响因子：
2.7
通讯作者：
Smith, James C.
中科院分区：
文献类型：
--
作者：
Callery, Elizabeth M.;Thomsen, Gerald H.;Smith, James C.
T-box家族转录因子在后生动物的发育中发挥着多种作用。在这里，我们描述了 Tbx6r，一种从两栖动物爪蟾中分离出来的独特的 Tbx6 旁系同源物。脊椎动物中这种不同的 T-box 基因的进化和发育整合揭示了这个保守的发育调节因子家族中意想不到的可塑性水平。我们发现，尽管 Tbx6 和 Tbx6r 共表达，但它们对配体处理的转录反应不同，并且它们激活配体表达的能力也非常不同。这两个旁系同源物具有不同的诱导特性：Tbx6 诱导中胚层，而 Tbx6r 诱导前神经标记物。我们使用混合蛋白来努力理解这种差异，并暗示蛋白质的 C 末端区域与其诱导特异性有关。通过使用反义吗啉代寡核苷酸进行功能丧失分析，我们发现 Tbx6 旁系同源物在非洲爪蟾近轴和中间中胚层以及神经嵴的发育中发挥重要功能。我们证明 Tbx6 和 Tbx6r 均诱导 FGF8 以及前基板标记物的表达，并且 Tbx6 还可以通过涉及 FGF8 和 Wnt8 的配体依赖性机制诱导神经嵴标记物。因此，我们的数据确定了这一关键发育调节因子的重要新功能。
T-box family transcription factors play many roles in Metazoan development. Here we characterise Tbx6r, a unique Tbx6 paralogue isolated from the amphibian Xenopus. The evolution and developmental integration of this divergent T-box gene within the vertebrates reveals an unexpected level of plasticity within this conserved family of developmental regulators. We show that despite their co-expression, Tbx6 and Tbx6r have dissimilar transcriptional responses to ligand treatment, and their ability to activate ligand expression is also very different. The two paralogues have distinct inductive properties: Tbx6 induces mesoderm whereas Tbx6r induces anterior neural markers. We use hybrid proteins in an effort to understand this difference, and implicate the C-terminal regions of the proteins in their inductive specificities. Through loss-of-function analyses using antisense morpholino oligonucleotides we show that both Tbx6 paralogues perform essential functions in the development of the paraxial and intermediate mesoderm and the neural crest in Xenopus. We demonstrate that Tbx6 and Tbx6r both induce FGF8 expression as well as that of pre-placodal markers, and that Tbx6 can also induce neural crest markers via a ligand-dependent mechanism involving FGF8 and Wnt8. Our data thus identify an important new function for this key developmental regulator.
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影响因子：
2.4
作者：
Adell, T;Grebenjuk, VA;Müller, WEG
通讯作者：
Müller, WEG
影响因子：
2.7
作者：
Chapman, DL;Agulnik, I;Papaioannou, VE
通讯作者：
Papaioannou, VE
影响因子：
--
作者：
Chesley, P
通讯作者：
Chesley, P
DOI：
10.1242/dev.026229
发表时间：
2008-12
期刊：
Development (Cambridge, England)
影响因子：
--
作者：
Hong CS;Park BY;Saint-Jeannet JP
通讯作者：
Saint-Jeannet JP
影响因子：
2.6
作者：
Chapman, DL;Cooper-Morgan, A;Papaioannou, VE
通讯作者：
Papaioannou, VE