Recruitment of vimentin to the cell surface by beta3 integrin and plectin mediates adhesion strength.
Recruitment of vimentin to the cell surface by beta3 integrin and plectin mediates adhesion strength.
复制标题
DOI：
10.1242/jcs.043042
复制
发表时间：
2009-05-01
影响因子：
4
通讯作者：
Jones JC
中科院分区：
文献类型：
--
作者：
Bhattacharya R;Gonzalez AM;Debiase PJ;Trejo HE;Goldman RD;Flitney FW;Jones JC
大量的努力已经花费在分析微丝和微管细胞骨架如何决定细胞与基质在称为粘着斑（FA）的粘附位点的相互作用。然而，波形蛋白中间丝（IF）也与内皮细胞中的FA处的细胞表面相关联。在这里，我们表明，IF招募到FA在内皮细胞需要β3整合素，plectin和微管细胞骨架，并依赖于微管马达。在缺乏β3整联蛋白但含有波形蛋白的CHO细胞中，IF似乎在细胞核周围塌陷，而在表达β3整联蛋白（CHOwtβ3）的CHO细胞中，波形蛋白IF延伸至细胞周边的FA。这种募集受β3整联蛋白胞质尾区中的酪氨酸残基调节。此外，CHOwtβ3细胞表现出比CHO或表达突变的β3整联蛋白的CHO细胞显著更大的粘附强度。这些差异需要完整的波形蛋白网络。因此，波形蛋白IF向细胞表面的募集受到严格调控，并调节细胞与其基质的粘附强度。
Much effort has been expended on analyzing how microfilament and microtubule cytoskeletons dictate the interaction of cells with matrix at adhesive sites called focal adhesions (FAs). However, vimentin intermediate filaments (IFs) also associate with the cell surface at FAs in endothelial cells. Here, we show that IF recruitment to FAs in endothelial cells requires β3 integrin, plectin and the microtubule cytoskeleton, and is dependent on microtubule motors. In CHO cells, which lack β3 integrin but contain vimentin, IFs appear to be collapsed around the nucleus, whereas in CHO cells expressing β3 integrin (CHOwtβ3), vimentin IFs extend to FAs at the cell periphery. This recruitment is regulated by tyrosine residues in the β3 integrin cytoplasmic tail. Moreover, CHOwtβ3 cells exhibit significantly greater adhesive strength than CHO or CHO cells expressing mutated β3 integrin proteins. These differences require an intact vimentin network. Therefore, vimentin IF recruitment to the cell surface is tightly regulated and modulates the strength of adhesion of cells to their substrate.
登录
查看更多内容
影响因子：
56.9
作者：
Ortiz-Urda, S;Garcia, J;Khavari, PA
通讯作者：
Khavari, PA
影响因子：
4.8
作者：
Schaffner-Reckinger, E;Gouon, V;Kieffer, N
通讯作者：
Kieffer, N
影响因子：
10.4
作者：
Hynes, R. O.
通讯作者：
Hynes, R. O.
影响因子：
3.3
作者：
Boettiger, D;Huber, F;Blystone, S
通讯作者：
Blystone, S
影响因子：
7.8
作者：
Prahlad, V;Yoon, M;Moir, R D;Vale, R D;Goldman, R D
通讯作者：
Goldman, R D