Disruption of the integrity and function of brain microvascular endothelial cells in culture by exposure to diesel engine exhaust particles.
Disruption of the integrity and function of brain microvascular endothelial cells in culture by exposure to diesel engine exhaust particles.
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DOI：
10.1016/j.toxlet.2013.03.023
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发表时间：
2013-06-20
影响因子：
3.5
通讯作者：
Ercal N
中科院分区：
文献类型：
--
作者：
Tobwala S;Zhang X;Zheng Y;Wang HJ;Banks WA;Ercal N
柴油机废气颗粒 (DEP) 是柴油发动机废气 (DEE) 的副产品，已知会产生促氧化和促炎症作用，从而导致氧化应激引起的损伤。鉴于 DEP 在诱导氧化应激中的关键作用，我们研究了 DEP 在破坏永生化人脑微血管内皮细胞 (HBMVEC) 完整性和功能中的作用。为了研究这一点，将 HBMVEC 细胞暴露于含有三种不同浓度 DEP 的培养基或普通培养基中 24 小时。谷胱甘肽 (GSH) 和丙二醛 (MDA) 水平以及谷胱甘肽过氧化物酶和谷胱甘肽还原酶活性表明，接触 DEP 的受试者比未处理组表现出显着更高的氧化应激。通过跨上皮电阻测量，DEP 还诱导 HBMVEC 细胞单层与氧化应激相关的破坏。总而言之，这些数据表明 DEP 诱导细胞死亡并破坏 HBMVEC 细胞的功能和完整性，表明 DEP 在神经毒性中的潜在作用。
Diesel exhaust particles (DEPs), a by-product of diesel engine exhaust (DEE), are known to produce pro-oxidative and pro-inflammatory effects, thereby leading to oxidative stress-induced damage. Given the key role of DEPs in inducing oxidative stress, we investigated the role of DEPs in disrupting the integrity and function of immortalized human brain microvascular endothelial cells (HBMVEC). To study this, HBMVEC cells were exposed to media containing three different concentrations of DEPs or plain media for 24 h. Those exposed to DEPs showed significantly higher oxidative stress than the untreated group, as indicated by the glutathione (GSH) and malondialdehyde (MDA) levels, and the glutathione peroxidase and glutathione reductase activities. DEPs also induced oxidative stress-related disruption of the HBMVEC cells monolayer, as measured by trans-epithelial electrical resistance. Taken together, these data suggest that DEPs induce cell death and disrupt the function and integrity of HBMVEC cells, indicating a potential role of DEPs in neurotoxicities.
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DOI：
10.1002/bio.1170020302
发表时间：
1988-07-01
期刊：
JOURNAL OF BIOLUMINESCENCE AND CHEMILUMINESCENCE
影响因子：
--
作者：
BELGHMI, K;NICOLAS, JC;DEPAULET, AC
通讯作者：
DEPAULET, AC
影响因子：
10
作者：
Lehmann, Andrea D.;Blank, Fabian;Rothen-Rutishauser, Barbara M.
通讯作者：
Rothen-Rutishauser, Barbara M.
影响因子：
6
作者：
Dallasta, LM;Pisarov, LA;Achim, CL
通讯作者：
Achim, CL
DOI：
10.1046/j.1365-2362.2002.00994.x
发表时间：
2002-05-01
影响因子：
5.5
作者：
Fiala, M;Liu, QN;Vinters, HV
通讯作者：
Vinters, HV
影响因子：
14.5
作者：
Bar-Or, A;Nuttall, RK;Yong, VW
通讯作者：
Yong, VW