Comparative cellular, physiological and transcriptome analyses reveal the potential easy dehulling mechanism of rice-tartary buckwheat (Fagopyrum Tararicum).
Comparative cellular, physiological and transcriptome analyses reveal the potential easy dehulling mechanism of rice-tartary buckwheat (Fagopyrum Tararicum).
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比较细胞、生理和转录组分析揭示了水稻-苦荞 (Fagopyrum Tararicum) 潜在的易脱壳机制
DOI：
10.1186/s12870-020-02715-7
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发表时间：
2020-11-04
影响因子：
5.3
通讯作者：
Chen QF
中科院分区：
文献类型：
--
作者：
Li HY;Wu CX;Lv QY;Shi TX;Chen QJ;Chen QF
研究背景苦荞因其营养丰富、黄酮类化合物含量高而在食品市场上受到广泛欢迎。然而，其困难的脱皮过程严重制约了其食品加工业的发展。水稻，苦荞麦，一种罕见的地方品种，是很容易脱皮，但这种容易脱皮的细胞，生理和分子机制仍然很不清楚。ResultsIn这项研究中，我们整合了比较细胞，生理，转录组和基因共表达网络的分析，深入了解的原因，水稻，苦荞麦是容易脱皮。与普通苦荞麦相比，水稻苦荞麦的船体明显变脆变薄，船体厚壁组织细胞壁明显变薄。苦荞船体的纤维素、半纤维素和木质素含量均显著低于所有或部分供试苦荞品种，且纤维素和半纤维素含量在授粉后10 d开始出现显著差异。比较转录组分析确定了9250差异表达基因（DEG）之间的水稻和正常的苦荞麦壳在四个不同的发展阶段。加权基因共表达网络分析（WGCNA）的所有DEG确定了一个关键模块与形成的船体之间的差异水稻和正常的苦荞。在这个特定的模块中，许多参与纤维素和半纤维素生物合成的次生细胞壁（SCW）生物合成调控和结构基因被鉴定为枢纽基因，并显示共表达。在船体发育的早期阶段，这些鉴定的SCW生物合成的枢纽基因在水稻-苦荞船体中的表达显著低于正常苦荞。其中17个SCW生物合成相关基因的表达进一步通过实时荧光定量PCR（qRT-PCR）得到验证。结论水稻-苦荞船体发育早期SCW生物合成调控基因和结构基因的表达较低，这进一步影响船体厚壁组织细胞的细胞壁厚度以及船体厚度和机械强度。
BackgroundTartary buckwheat has gained popularity in the food marketplace due to its abundant nutrients and high bioactive flavonoid content. However, its difficult dehulling process has severely restricted its food processing industry development. Rice-tartary buckwheat, a rare local variety, is very easily dehulled, but the cellular, physiological and molecular mechanisms responsible for this easy dehulling remains largely unclear.ResultsIn this study, we integrated analyses of the comparative cellular, physiological, transcriptome, and gene coexpression network to insight into the reason that rice-tartary buckwheat is easy to dehull. Compared to normal tartary buckwheat, rice-tartary buckwheat has significantly brittler and thinner hull, and thinner cell wall in hull sclerenchyma cells. Furthermore, the cellulose, hemicellulose, and lignin contents of rice-tartary buckwheat hull were significantly lower than those in all or part of the tested normal tartary buckwheat cultivars, respectively, and the significant difference in cellulose and hemicellulose contents between rice-tartary buckwheat and normal tartary buckwheat began at 10 days after pollination (DAP). Comparative transcriptome analysis identified a total of 9250 differentially expressed genes (DEGs) between the rice- and normal-tartary buckwheat hulls at four different development stages. Weighted gene coexpression network analysis (WGCNA) of all DEGs identified a key module associated with the formation of the hull difference between rice- and normal-tartary buckwheat. In this specific module, many secondary cell wall (SCW) biosynthesis regulatory and structural genes, which involved in cellulose and hemicellulose biosynthesis, were identified as hub genes and displayed coexpression. These identified hub genes of SCW biosynthesis were significantly lower expression in rice-tartary buckwheat hull than in normal tartary buckwheat at the early hull development stages. Among them, the expression of 17 SCW biosynthesis relative-hub genes were further verified by quantitative real-time polymerase chain reaction (qRT-PCR).ConclusionsOur results showed that the lower expression of SCW biosynthesis regulatory and structural genes in rice-tartary buckwheat hull in the early development stages contributes to its easy dehulling by reducing the content of cell wall chemical components, which further effects the cell wall thickness of hull sclerenchyma cells, and hull thickness and mechanical strength.
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WGCNA：用于加权相关网络分析的 R 包。
影响因子：
3
作者：
Langfelder P;Horvath S
通讯作者：
Horvath S
影响因子：
48
作者：
Langmead, Ben;Salzberg, Steven L.
通讯作者：
Salzberg, Steven L.
影响因子：
8.1
作者：
Dziedzic, Krzysztof;Gorecka, Danuta;Przybylska, Barbara
通讯作者：
Przybylska, Barbara
影响因子：
11.6
作者：
Mitsuda, N;Seki, M;Ohme-Takagi, M
通讯作者：
Ohme-Takagi, M
苦荞种壳厚度与产量因子的相关性及遗传分析
影响因子：
2.4
作者：
Li, Chunhua;Xie, Zhiming;Wang, Lihua
通讯作者：
Wang, Lihua