Calpain 2 is required for glioblastoma cell invasion: regulation of matrix metalloproteinase 2.
Calpain 2 is required for glioblastoma cell invasion: regulation of matrix metalloproteinase 2.
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DOI：
10.1007/s11064-010-0246-8
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发表时间：
2010-11
影响因子：
4.4
通讯作者：
Greenwood, Jeffrey A.
中科院分区：
文献类型：
--
作者：
Jang, Hyo Sang;Lal, Sangeet;Greenwood, Jeffrey A.
胶质母细胞瘤细胞的侵袭显著降低了目前治疗的有效性，突出了了解侵袭性群体的扩散机制和特征的重要性。自分泌谷氨酸诱导钙流入胶质母细胞瘤细胞是侵袭的关键。然而，钙刺激胶质母细胞瘤细胞扩散的靶点尚不清楚。在这项研究中，我们测试的假设，钙激活蛋白酶钙蛋白酶2是胶质母细胞瘤细胞侵袭所必需的。使用shRNA敲低钙蛋白酶2表达或化学抑制钙蛋白酶活性可使胶质母细胞瘤细胞侵袭减少90%。有趣的是，钙蛋白酶2的表达减少并不影响形态学或迁移，这表明调节入侵的具体机制。与这一想法一致，从敲低细胞中测量到39%的细胞外MMP 2减少，鉴定了钙蛋白酶2介导胶质母细胞瘤细胞侵袭的一种机制。这是第一份证明钙蛋白酶2是胶质母细胞瘤细胞侵袭所必需的报告。
Invasion of glioblastoma cells significantly reduces the effectiveness of current treatments, highlighting the importance of understanding dispersal mechanisms and characteristics of the invasive population. Induction of calcium fluxes into glioblastoma cells by autocrine glutamate is critical for invasion. However, the target(s) by which calcium acts to stimulate the dispersal of glioblastoma cells is not clear. In this study, we tested the hypothesis that the calcium-activated protease calpain 2 is required for glioblastoma cell invasion. Knockdown of calpain 2 expression using shRNA or chemical inhibition of calpain activity reduced glioblastoma cell invasion by 90%. Interestingly, decreased expression of calpain 2 did not influence morphology or migration, suggesting regulation of invasion specific mechanisms. Consistent with this idea, 39% less extracellular MMP2 was measured from knockdown cells identifying one mechanism by which calpain 2 mediates glioblastoma cell invasion. This is the first report demonstrating that calpain 2 is required for glioblastoma cell invasion.
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影响因子：
4.8
作者：
Glading, A;Chang, P;Wells, A
通讯作者：
Wells, A
影响因子：
11.2
作者：
Libertini, SJ;Robinson, BS;Mudryj, M
通讯作者：
Mudryj, M
影响因子：
7.5
作者：
Gimona, Mario;Buccione, Roberto;Linder, Stefan
通讯作者：
Linder, Stefan
DOI：
10.1083/jcb.200708048
发表时间：
2008-03-10
期刊：
The Journal of cell biology
影响因子：
--
作者：
Cortesio CL;Chan KT;Perrin BJ;Burton NO;Zhang S;Zhang ZY;Huttenlocher A
通讯作者：
Huttenlocher A
影响因子：
5.4
作者：
Huber, E;Vlasny, D;Iftner, T
通讯作者：
Iftner, T