Histochemical and autoradiographic studies on elcatonin internalization and intracellular movement in osteoclasts
Histochemical and autoradiographic studies on elcatonin internalization and intracellular movement in osteoclasts
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破骨细胞中降钙素内化和细胞内运动的组织化学和放射自显影研究
DOI：
10.1002/jbmr.5650090105
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发表时间：
1994
影响因子：
6.2
通讯作者：
H. Ozawa
中科院分区：
文献类型：
--
作者：
M. Ikegame;S. Ejiri;H. Ozawa
使用[125 I]依卡宁（125 I-eCT）通过放射自显影检查破骨细胞中依卡宁（eCT）的结合位点和时间定位。除了迄今报道的降钙素（CT）引起的结构变化外，还观察到高尔基体结构的变化。这些变化持续至与eCT孵育后48-72 h。125 I-eCT的发达银颗粒定位于多核破骨细胞和单核细胞中，这些细胞在超微结构上被定义为“前破骨细胞”。银颗粒位于这些细胞的质膜上，然后被内化;它们积累，特别是在高尔基体中，并保持48-72 h。在溶酶体和小泡中也检测到少量银颗粒。高尔基体中银颗粒数量的减少伴随着破骨细胞结构的恢复-高尔基体，然后是皱褶边缘。结果提示：（1）CT对破骨细胞高尔基体的分选功能有明显的抑制作用，使CT在高尔基体中滞留时间延长; (2)高尔基体中的CT可能保持其活性，并使CT对破骨细胞活性的影响延长。
The binding sites and chronologic localization of elcatonin (eCT) in osteoclasts were examined by autoradiography using [125I]elcatonin (125I‐eCT). In addition to the structural changes induced by calcitonin (CT) reported so far, changes were also observed in the structure of Golgi apparatus. These changes continued until 48–72 h after incubation with eCT. Developed silver grains of 125I‐eCT were localized into multinucleated osteoclasts and mononuclear cells that were ultrastructurally defined as “preosteoclasts.” The silver grains located on plasma membranes of those cells and were then internalized; they accumulated, especially in the Golgi apparatus, and remained for 48–72 h. A few silver grains were also detected in lysosomes and small vesicles. The decrease in the number of silver grains in the Golgi apparatus accompanied the recovery of osteoclast structures – Golgi apparatus and then ruffled borders. These findings suggest that (1) CT especially inhibits the sorting function of Golgi apparatus in osteoclasts, resulting in prolonged retention of CT in this organelle. (2) The CT in Golgi apparatus may keep its activity and cause the prolonged effect of CT on osteoclast activity.
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DOI：
10.1177/37.10.2778308
发表时间：
1989
期刊：
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society
影响因子：
--
作者：
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通讯作者：
Gay,CV
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DOI：
10.1016/0169-6009(91)90068-b
发表时间：
1991
期刊：
Bone and mineral
影响因子：
--
作者：
Minkin,C;Yu,XH
通讯作者：
Yu,XH
降钙素灭活破骨细胞期间，顶端质膜的选择性内化以及溶酶体酶和 6-磷酸甘露糖受体的快速重新分布。
影响因子：
4
作者：
Baron,R;Neff,L;Brown,W;Louvard,D;Courtoy,PJ
通讯作者：
Courtoy,PJ
影响因子：
56.9
作者：
LIN, HY;HARRIS, TL;GOLDRING, SR
通讯作者：
GOLDRING, SR