Integrative genome-wide approaches in embryonic stem cell research.
Integrative genome-wide approaches in embryonic stem cell research.
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DOI：
10.1039/c0ib00068j
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发表时间：
2010-10
期刊：
影响因子：
--
通讯作者：
Huang J
中科院分区：
文献类型：
--
作者：
Zhang X;Huang J
胚胎干（ES）细胞来源于胚泡。它们可以分化成三个胚层和基本上任何类型的体细胞。因此，它们在组织再生治疗中具有巨大的潜力。从各种类型的体细胞产生诱导多能干细胞（iPS）的技术突破解决了与使用人类胚胎干细胞相关的伦理问题。然而，ES和iPS细胞如何自我更新和保持其多能性仍然是未知的，尽管在过去的二十年中已经取得了巨大的进展。基因表达微阵列、基于染色质免疫沉淀的微阵列（ChIP-chip）和染色质免疫沉淀后大规模平行测序（ChIP-seq）等全基因组整合方法为阐明ES和iPS细胞的多能性、重编程和DNA损伤反应机制提供了前所未有的机会。本文综述了ES细胞和iPS细胞的基本生物学问题，并综述了利用全基因组技术研究ES细胞和iPS细胞的最新进展。最后，我们对干细胞全基因组研究的未来提出了自己的观点。
Embryonic stem (ES) cells are derived from blastocysts. They can differentiate into the three embryonic germ layers and essentially any type of somatic cells. Therefore, they hold great potentials in tissue regeneration therapy. The ethical issues associated with the use of human embryonic stem cells are resolved by the technical break-through of generating induced pluripotent stem (iPS) cells from various types of somatic cells. However, how ES and iPS cells self-renew and maintain their pluripotency is still largely unknown in spite of the great progresses that have been made in the last two decades. Integrative genome-wide approaches, such as gene expression microarray, chromatin immunoprecipitation based microarray (ChIP-chip) and chromatin immunoprecipitation followed by massive parallel sequencing (ChIP-seq) offer unprecedented opportunities to elucidate the mechanism of the pluripotency, reprogramming and DNA damage response of ES and iPS cells. This review summarized the fundamental biological questions about ES and iPS cells and reviewed the recent advances in ES and iPS cell research using genome-wide technologies. In the end, we offered our perspectives on the future of genome-wide studies on stem cells.
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DOI：
10.1039/b814329c
发表时间：
2009-01
期刊：
Integrative biology : quantitative biosciences from nano to macro
影响因子：
--
作者：
Irimia D;Mindrinos M;Russom A;Xiao W;Wilhelmy J;Wang S;Heath JD;Kurn N;Tompkins RG;Davis RW;Toner M
通讯作者：
Toner M
影响因子：
64.5
作者：
Goldberg AD;Banaszynski LA;Noh KM;Lewis PW;Elsaesser SJ;Stadler S;Dewell S;Law M;Guo X;Li X;Wen D;Chapgier A;DeKelver RC;Miller JC;Lee YL;Boydston EA;Holmes MC;Gregory PD;Greally JM;Rafii S;Yang C;Scambler PJ;Garrick D;Gibbons RJ;Higgs DR;Cristea IM;Urnov FD;Zheng D;Allis CD
通讯作者：
Allis CD
影响因子：
10.5
作者：
Cole, Megan F.;Johnstone, Sarah E.;Young, Richard A.
通讯作者：
Young, Richard A.
DOI：
10.1073/pnas.0909734107
发表时间：
2010-01-05
影响因子：
11.1
作者：
Lee, Kyoung-Hwa;Li, Mangmang;Huang, Jing
通讯作者：
Huang, Jing
影响因子：
64.5
作者：
Kim, Jonghwan;Chu, Jianlin;Orkin, Stuart H.
通讯作者：
Orkin, Stuart H.