Efficient in vivo vascularization of tissue-engineering scaffolds.
Efficient in vivo vascularization of tissue-engineering scaffolds.
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DOI：
10.1002/term.336
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发表时间：
2011-04
影响因子：
3.3
通讯作者：
Lorens, James B.
中科院分区：
文献类型：
--
作者：
Hegen, Anja;Blois, Anna;Tiron, Crina E.;Hellesoy, Monica;Micklem, David R.;Nor, Jacques E.;Akslen, Lars A.;Lorens, James B.
组织工程的成功取决于功能性血管系统的快速有效形成。成人血管包括内皮细胞和血管周壁细胞，其在血管生成期间组装成由基底膜包裹的未闭小管。使用单独的血管成分，我们的特点是支架内微血管自组装效率在生理体内组织工程植入的背景。将原代人微血管内皮细胞和血管平滑肌细胞以不同比例接种在富含基底膜蛋白（Matrigel）的聚-L乳酸（PLLA）支架中，并皮下植入免疫功能低下的小鼠。通过免疫组化、流式细胞术和体内多光子荧光显微镜分析监测支架内颞叶微血管的形成、吻合和灌注。组织工程背景下的血管化在接种了完整的血管成分的植入物中得到了强烈的增强：人微血管内皮细胞和血管平滑肌细胞在体内在富含基质胶的PLLA支架内组装了一个专利的微血管系统，该支架在植入的第一周期间与宿主循环结合。支架内微循环的多光子荧光血管造影分析显示了均匀的分支微血管网络。血管化植入物内hPASMC分布的3-D图像重建分析是非随机的，并显示出优先的血管周围定位。因此，有效的微血管自组装，吻合和建立一个功能性的微血管培养在天然缺氧的体内组织工程的背景下，通过提供一套完整的血管组件。
The success of tissue engineering depends on the rapid and efficient formation of a functional blood vasculature. Adult blood vessels comprise endothelial cells and peri-vascular mural cells that assemble into patent tubules ensheathed by a basement membrane during angiogenesis. Using individual vessel components, we characterized intra-scaffold microvessel self-assembly efficiency in a physiological in vivo tissue engineering implant context. Primary human microvascular endothelial- and vascular smooth muscle cells were seeded at different ratios in poly-L lactic acid (PLLA) scaffolds enriched with basement membrane proteins (Matrigel) and implanted subcutaneously into immunocompromised mice. Temporal intra-scaffold microvessel formation, anastomosis and perfusion were monitored by immunohistochemical, flow cytometric and in vivo multiphoton fluorescence microscopy analysis. Vascularization in the tissue engineering context was strongly enhanced in the implants seeded with a complete complement of blood vessel components: Human microvascular endothelial and vascular smooth muscle cells in vivo assembled a patent microvasculature within Matrigel-enriched PLLA scaffolds that anastomosed with the host circulation during the first week of implantation. Multiphoton fluorescence angiographic analysis of the intra-scaffold microcirculation showed a uniform, branched microvascular network. 3-D image reconstruction analysis of hPASMC distribution within vascularized implants was non-random and displayed a preferential peri-vascular localization. Hence, efficient microvessel self-assembly, anastomosis and establishment of a functional microvasculture in the native hypoxic in vivo tissue engineering context is promoted by providing a complete set of vascular components.
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影响因子：
4.8
作者：
Korff, T;Kimmina, S;Augustin, HG
通讯作者：
Augustin, HG
影响因子：
3.7
作者：
Evensen, Lasse;Micklem, David R.;Lorens, James B.
通讯作者：
Lorens, James B.
影响因子：
11.2
作者：
Holland, SJ;Powell, MJ;Lorens, JB
通讯作者：
Lorens, JB
DOI：
10.1073/pnas.0909333107
发表时间：
2010-01-19
影响因子：
11.1
作者：
Gjerdrum, Christine;Tiron, Crina;Lorens, James B.
通讯作者：
Lorens, James B.
影响因子：
6.4
作者：
Kaully, Tamar;Kaufman-Francis, Keren;Levenberg, Shulamit
通讯作者：
Levenberg, Shulamit