Biosensors for qualitative and semiquantitative analysis of quorum sensing signal molecules.

Biosensors for qualitative and semiquantitative analysis of quorum sensing signal molecules.
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用于群体感应信号分子的定性和半定量分析的生物传感器。

DOI:
10.1007/978-1-4939-0473-0_20
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发表时间:
2014
期刊:
Methods in molecular biology (Clifton, N.J.)
影响因子:
--
通讯作者:
Fletcher M
Fletcher M
中科院分区:
--
文献类型:
--
作者:
Fletcher M

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生物传感器是一种生物工具,可用于定量和半定量地测定细菌培养物的群体感应信号分子(QSSMs)。QSSMs可以用有机溶剂从铜绿假单胞菌培养基中提取,并通过结合生物传感器覆盖层的薄层色谱法初步鉴定。或者,可以使用基于生物传感器的分光光度法、发光法或荧光法,在废培养上清液或溶剂提取物中对QSSMs进行定量。
Biosensors are biological tools that can be used to assay bacterial cultures for quorum sensing signal molecules (QSSMs) both qualitatively and semiquantitatively. QSSMs can be extracted fromPseudomonas aeruginosacultures using organic solvents and tentatively identified via thin layer chromatography in combination with biosensor overlays. Alternatively, QSSMs can be quantified in spent culture supernatants or solvent extracts using biosensor-based spectrophotometric, luminescence, or fluorescence assays.
DOI: 10.1038/nprot.2007.158
发表时间: 2007-01-01
期刊: NATURE PROTOCOLS
影响因子: 14.8
作者:
Fletcher, Matthew P.;Diggle, Stephen P.;Williams, Paul
通讯作者: Williams, Paul
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DOI: 10.1007/s00253-012-4236-4
发表时间: 2013
影响因子: 5
作者:
Müller C;Fetzner S
通讯作者: Fetzner S