Specific cleavage of the large subunit of replication factor C in apoptosis is mediated by CPP32-like protease.
Specific cleavage of the large subunit of replication factor C in apoptosis is mediated by CPP32-like protease.
复制标题
细胞凋亡中复制因子 C 大亚基的特异性切割是由 CPP32 样蛋白酶介导的。
DOI：
10.1006/bbrc.1997.6456
复制
发表时间：
1997
影响因子：
3.1
通讯作者：
M. Lavin
中科院分区：
文献类型：
--
作者：
Q. Song;H. Lu;N. Zhang;B. Luckow;G. Shah;G. Poirier;M. Lavin
最近的证据表明，与白细胞介素-1 β转化酶（ICE）相关的半胱氨酸蛋白酶家族在介导细胞凋亡中具有重要作用。已经报道了这些酶对一小组细胞底物的蛋白水解裂解与细胞凋亡的发生有关。在本研究中，我们在蛋白质数据库中搜索了具有CPP 32切割位点DEVD的潜在死亡底物，并鉴定了几种候选物，包括RFC 140，复制因子C的大亚基，我们随后证明其在响应于不同细胞毒性剂而经历凋亡的各种细胞类型中特异性切割，而在对依托泊苷诱导的细胞凋亡有抗性的细胞系中没有观察到降解。CPP 32的有效抑制剂Ac-DEVD-CHO对凋亡提取物中RFC 140切割的消除，以及CPP 32共有切割序列DEVD存在于RFC 140中的发现，表明CPP 32或其近亲负责细胞凋亡中的RFC 140降解。
Recent evidence suggests that the growing family of cysteine proteases related to the interleukin-1beta-converting enzyme (ICE) is of central importance in mediating apoptosis. Proteolytic cleavage of a small group of cellular substrates by these enzymes in association with the onset of apoptosis has been reported. In the present study, we searched a protein data base for potential death substrates possessing the CPP32 cleavage site, DEVD, and identified several candidates including RFC140, the large subunit of replication factor C, which we subsequently demonstrated to be specifically cleaved in a variety of cell types undergoing apoptosis in response to different cytotoxic agents, whereas no degradation is observed in a cell line resistant to etoposide-induced apoptosis. The abrogation of RFC140 cleavage in apoptotic extracts by Ac-DEVD-CHO, a potent inhibitor of CPP32, together with the finding that a CPP32 consensus cleavage sequence, DEVD, exists in RFC140, suggests that CPP32 or a close relative is responsible for RFC140 degradation in apoptosis.
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用纯化的蛋白质重建猿猴病毒 40 DNA 复制。
DOI：
10.1073/pnas.87.22.8692
发表时间：
1990
影响因子：
11.1
作者：
Weinberg,DH;Collins,KL;Simancek,P;Russo,A;Wold,MS;Virshup,DM;Kelly,TJ
通讯作者：
Kelly,TJ
DOI：
10.1016/s0021-9258(18)47344-9
发表时间：
1994-12
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
T. Fernandes‐Alnemri;G. Litwack;E. Alnemri
通讯作者：
T. Fernandes‐Alnemri;G. Litwack;E. Alnemri
影响因子：
10.5
作者：
KUMAR, S;KINOSHITA, M;JENKINS, NA
通讯作者：
JENKINS, NA
DOI：
10.1016/s0021-9258(18)52476-5
发表时间：
1991-01
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Suk-Hee Lee;Ann D. Kwong;Zhen-Qiang Pan;J. Hurwitz
通讯作者：
Suk-Hee Lee;Ann D. Kwong;Zhen-Qiang Pan;J. Hurwitz
DOI：
10.1073/pnas.90.23.11014
发表时间：
1993-12-01
影响因子：
11.1
作者：
BUNZ, F;KOBAYASHI, R;STILLMAN, B
通讯作者：
STILLMAN, B