Global linkage map connects meiotic centromere function to chromosome size in budding yeast.
Global linkage map connects meiotic centromere function to chromosome size in budding yeast.
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DOI：
10.1534/g3.113.007377
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发表时间：
2013-10-03
期刊：
影响因子：
--
通讯作者：
Boone C
中科院分区：
文献类型：
--
作者：
Baryshnikova A;VanderSluis B;Costanzo M;Myers CL;Cha RS;Andrews B;Boone C
合成遗传阵列（SGA）分析使酵母遗传学自动化，能够高通量构建双突变体的有序阵列。从SGA分析得到的定量菌落大小可用于测量细胞适合度和遗传相互作用的评分，例如合成致死率。在这里，我们表明，SGA菌落大小也可以用来获得减数分裂重组的全球地图，因为重组频率影响双突变体的形成位于同一染色体上的基因对，因此影响所得到的双突变体菌落的大小。我们获得了位于同一染色体上的约120万个双突变体的定量菌落大小数据，并构建了约5 kb分辨率的基因组规模遗传连锁图谱。我们发现，我们的连锁图谱是可重复的，并与以前的全球减数分裂重组的研究一致。特别是，我们证实，每个染色体的总交叉数往往遵循一个简单的线性模型，取决于染色体的大小。此外，我们观察到一个以前不受重视的连锁区域的大小之间的关系，围绕每个着丝粒和染色体的大小，这表明，交叉往往发生在更远离着丝粒较大的染色体。较大染色体的臂间区也出现负载较大的减数分裂凝聚素Rec8的集群，并获得较少的Spo11催化的DNA双链断裂。考虑到离着丝粒太近或太远的交换不利于同源分离并增加非整倍体的发生率，我们的数据表明染色体大小可能在减数分裂期间调节染色体分离的保真度中起直接作用。
Synthetic genetic array (SGA) analysis automates yeast genetics, enabling high-throughput construction of ordered arrays of double mutants. Quantitative colony sizes derived from SGA analysis can be used to measure cellular fitness and score for genetic interactions, such as synthetic lethality. Here we show that SGA colony sizes also can be used to obtain global maps of meiotic recombination because recombination frequency affects double-mutant formation for gene pairs located on the same chromosome and therefore influences the size of the resultant double-mutant colony. We obtained quantitative colony size data for ~1.2 million double mutants located on the same chromosome and constructed a genome-scale genetic linkage map at ~5 kb resolution. We found that our linkage map is reproducible and consistent with previous global studies of meiotic recombination. In particular, we confirmed that the total number of crossovers per chromosome tends to follow a simple linear model that depends on chromosome size. In addition, we observed a previously unappreciated relationship between the size of linkage regions surrounding each centromere and chromosome size, suggesting that crossovers tend to occur farther away from the centromere on larger chromosomes. The pericentric regions of larger chromosomes also appeared to load larger clusters of meiotic cohesin Rec8, and acquire fewer Spo11-catalyzed DNA double-strand breaks. Given that crossovers too near or too far from centromeres are detrimental to homolog disjunction and increase the incidence of aneuploidy, our data suggest that chromosome size may have a direct role in regulating the fidelity of chromosome segregation during meiosis.
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映射减数分裂的单链DNA揭示了酿酒酵母中DNA双链断裂的新景观。
影响因子：
9.8
作者：
Buhler, Cyril;Borde, Valerie;Lichten, Michael
通讯作者：
Lichten, Michael
影响因子：
64.5
作者：
Blat, Y;Kleckner, N
通讯作者：
Kleckner, N
影响因子：
9.8
作者：
Glynn, EF;Megee, PC;Gerton, JL
通讯作者：
Gerton, JL
在基因组量表上对酵母中适应性和遗传相互作用的定量分析。
影响因子：
48
作者：
Baryshnikova, Anastasia;Costanzo, Michael;Kim, Yungil;Ding, Huiming;Koh, Judice;Toufighi, Kiana;Youn, Ji-Young;Ou, Jiongwen;San Luis, Bryan-Joseph;Bandyopadhyay, Sunayan;Hibbs, Matthew;Hess, David;Gingras, Anne-Claude;Bader, Gary D.;Troyanskaya, Olga G.;Brown, Grant W.;Andrews, Brenda;Boone, Charles;Myers, Chad L.
通讯作者：
Myers, Chad L.
影响因子：
12.3
作者：
Collins SR;Schuldiner M;Krogan NJ;Weissman JS
通讯作者：
Weissman JS