MicroRNA Dysregulation and Steroid Hormone Receptor Expression in Uterine Tissues of Rats with Endometriosis during the Implantation Window.
MicroRNA Dysregulation and Steroid Hormone Receptor Expression in Uterine Tissues of Rats with Endometriosis during the Implantation Window.
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植入窗期子宫内膜异位症大鼠子宫组织中microRNA失调和类固醇激素受体表达
DOI：
10.4103/0366-6999.240808
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发表时间：
2018-09-20
影响因子：
6.1
通讯作者：
Lang JH
中科院分区：
文献类型：
--
作者：
Cai H;Zhu XX;Li ZF;Zhu YP;Lang JH
雌激素受体（ER）和孕激素受体（PR）与子宫内膜异位症有关，但 microRNA（miRNA）的参与情况尚不清楚。本研究的目的是探讨子宫内膜异位症大鼠着床窗口期子宫组织中 miRNA 与 ER/PR 的相关性。将 20 只雌性 Sprague-Dawley 大鼠随机分为三组：子宫内膜异位症组 (n = 7)、脂肪组织对照组 (n = 6) 和正常组 (n = 7)。雌性大鼠在第5天（着床）交配并处死。获得子宫组织用于苏木精-伊红染色、免疫组织化学和miRNA表达。使用逆转录聚合酶链反应（RT-PCR）来验证rno-miR-29c-3p、rno-miR-34c-5p、rno-miR-141-5p、rno-miR-24-1-5p和rno-miR-490-5p的表达。发现子宫内膜异位症组和正常对照组之间存在差异表达的 475 个 miRNA，其中 127 个 miRNA 上调，348 个 miRNA 下调。通过 RT-PCR 验证了 5 种 miRNA（rno-miR-29c-3p、rno-miR-34c-5p、rno-miR-141-5p、rno-miR-24-1-5p 和 rno-miR-490-5p）的表达，发现三组之间表达存在差异。三组间腺上皮和子宫内膜间质中 ER 和 PR 蛋白的表达（免疫组织化学）存在显着差异（均 P < 0.05）。五种 miRNA 参与可能参与着床和生育的途径。结果提示miRNA、ER、PR在子宫内膜异位症大鼠胚胎着床期发挥重要作用。这些 miRNA 可能在子宫内膜异位症的子宫内膜容受性中发挥作用。
Estrogen receptor (ER) and progesterone receptor (PR) are involved in endometriosis, but the involvement of microRNAs (miRNAs) is unknown. The aim of the study was to explore the correlation between miRNA and ER/PR in uterine tissues of rats with endometriosis during the implantation window. Twenty female Sprague-Dawley rats were randomized in three groups: endometriosis (n = 7), fat tissue control (n = 6), and normal (n = 7) groups. The female rats were mated and sacrificed on day 5 (implantation). Uterine tissues were obtained for hematoxylin-eosin staining, immunohistochemistry, and miRNA expression. Reverse transcription polymerase chain reaction (RT-PCR) was used to validate the expression of rno-miR-29c-3p, rno-miR-34c-5p, rno-miR-141-5p, rno-miR-24-1-5p, and rno-miR-490-5p. The 475 miRNAs were found to differentially express between the endometriosis and normal control groups, with 127 being upregulated and 348 being downregulated. Expression of five miRNAs (rno-miR-29c-3p, rno-miR-34c-5p, rno-miR-141-5p, rno-miR-24-1-5p, and rno-miR-490-5p) were validated by RT-PCR and found to be differentially expressed among the three groups. Expression of ER and PR proteins (immunohistochemistry) in the glandular epithelium and endometrial stroma was significantly different among the three groups (all P < 0.05). Five miRNAs were involved in pathways probably taking part in implantation and fertility. The results suggested that miRNAs, ER, and PR could play important roles in the embryo implantation period of rats with endometriosis. These miRNAs might play a role in endometrial receptivity in endometriosis.
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影响因子：
8
作者：
通讯作者：
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mmu-miR-141 在胚胎植入后早期妊娠小鼠子宫内膜中的可能作用
影响因子：
3.7
作者：
Liu X;Gao R;Chen X;Zhang H;Zheng A;Yang D;Ding Y;Wang Y;He J
通讯作者：
He J
影响因子：
3.5
作者：
Kropp, Jenna;Khatib, Hasan
通讯作者：
Khatib, Hasan
影响因子：
5.4
作者：
Long, Mei;Wan, Xiaohui;Cai, Xia
通讯作者：
Cai, Xia
影响因子：
11.4
作者：
Mallory, AC;Reinhart, BJ;Bartel, DP
通讯作者：
Bartel, DP