Possible roles of mmu-miR-141 in the endometrium of mice in early pregnancy following embryo implantation.
Possible roles of mmu-miR-141 in the endometrium of mice in early pregnancy following embryo implantation.
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mmu-miR-141 在胚胎植入后早期妊娠小鼠子宫内膜中的可能作用
DOI：
10.1371/journal.pone.0067382
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
He J
中科院分区：
文献类型：
--
作者：
Liu X;Gao R;Chen X;Zhang H;Zheng A;Yang D;Ding Y;Wang Y;He J
目的子宫接受性相关基因直接影响胚胎着床。研究已经证明了mirna在基因表达调控中的重要作用。我们的早期miRNA芯片分析显示，胚胎着床后子宫内膜组织中mmu-miR-141的表达低于胚胎着床前。然而，miR-141在胚胎着床中的可能作用尚未被阐明。在这里，我们设计了mmu-miR-141来检测miR-141在胚胎着床后早期妊娠小鼠子宫内膜中的表达和作用。方法采用real -time PCR和原位杂交技术检测小鼠子宫中mum - mir -141的表达。采用四氮唑染色法（MTT）和流式细胞术检测细胞增殖情况。采用Real-time PCR和Western blot分析确认磷酸酶和紧张素同源物（PTEN） mRNA和蛋白水平，以确定其是否为mmu-miR-141的靶基因。增强绿色荧光蛋白（EGFP）荧光报告载体分析。通过向小鼠子宫注射mmu-miR-141抑制剂或模拟载体进行功能研究。结果smmu- mir -141的表达在第6天（D6）低于第4天（D4），并可通过孕激素升高。降低mmu-miR-141可降低基质细胞的增殖活性，促进细胞凋亡。上调mmu-miR-141抑制PTEN蛋白表达，下调mmu-miR-141增加PTEN蛋白表达，而mRNA水平保持不变。EGFP荧光报告载体分析显示，miR-141靶向PTEN mRNA的3 ' -未翻译区。此外，当通过注射抑制剂或模拟物改变D2上的生生性mum - mir -141水平时，D7上的胚胎着床部位明显减少。结论mmu-miR-141可能通过负向调控PTEN的表达影响子宫内膜细胞增殖和凋亡，并影响胚胎着床位点的数量。mmu-miR-141在胚胎着床中起着至关重要的作用。
ObjectiveEmbryo implantation is directly affected by genes related to uterine receptivity. Studies have demonstrated the important roles of miRNAs in the regulation of gene expression. Our early miRNA chip analyses revealed that the mmu-miR-141 expression in endometrial tissue is lower after embryo implantation than before it. However, the possible roles of miR-141 in embryo implantation have not yet been elucidated. Here, mmu-miR-141 was designed to detect the expression and role of miR-141 in the endometria of mice in early pregnancy following embryo implantation.MethodsReal-time PCR and in-situ hybridization were used to study mmu-miR-141 expression in mouse uterus. Cell proliferation was detected by tetrazolium dye (MTT) assay and flow cytometry. Real-time PCR and Western blot analysis were used to confirm the mRNA and protein levels of phosphatase and tensin homolog (PTEN) to determine whether it was the target gene of mmu-miR-141. Enhanced green fluorescent protein (EGFP) fluorescence reporter vector analysis was also performed. A functional study was performed by injecting mice uteri with mmu-miR-141 inhibitor or mimic vectors.Resultsmmu-miR-141 expression was lower on day 6 (D6) than day 4 (D4) and could be increased by progesterone. Reduced mmu-miR-141 could decrease the proliferation activity of stromal cells and promote apoptosis. Upregulation of mmu-miR-141 inhibited PTEN protein expression but downregulation of mmu-miR-141 increased it, while the mRNA level remained unchanged. EGFP fluorescence reporter vector analysis showed that miR-141 targets the 3′-untranslated region of the PTEN mRNA. In addition, when the physiological mmu-miR-141 level was altered on D2 by injecting with inhibitor or mimic, the embryo implantation sites were significantly decreased on D7.ConclusionsThis study demonstrated that mmu-miR-141 might influence cell proliferation and apoptosis in the endometrium by negatively regulating PTEN expression, and could also influence the number of embryo implantation sites. mmu-miR-141 plays an essential role in embryo implantation.
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影响因子：
4.8
作者：
Eades, Gabriel;Yao, Yuan;Zhou, Qun
通讯作者：
Zhou, Qun
小鼠子宫胚胎着床过程中MicroRNA的表达和调控
影响因子：
4.8
作者：
Hu, Shi-Jun;Ren, Gang;Yang, Zeng-Ming
通讯作者：
Yang, Zeng-Ming
子宫内膜癌中PTEN表达与PI3K/Akt信号通路的相关性
DOI：
10.1007/s11596-009-0112-6
发表时间：
2009-02-01
影响因子：
--
作者：
Gao, Qinglei;Ye, Fei;Ma, Ding
通讯作者：
Ma, Ding
影响因子：
--
作者：
Agaoglu, Fulya Yaman;Kovancilar, Muge;Gezer, Ugur
通讯作者：
Gezer, Ugur
影响因子：
3.4
作者：
Chegini, N;Ma, CF;Ripps, BA
通讯作者：
Ripps, BA