Enzymatic control of anhydrobiosis-related accumulation of trehalose in the sleeping chironomid, Polypedilum vanderplanki.
Enzymatic control of anhydrobiosis-related accumulation of trehalose in the sleeping chironomid, Polypedilum vanderplanki.
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DOI：
10.1111/j.1742-4658.2010.07811.x
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发表时间：
2010-10
期刊：
影响因子：
--
通讯作者：
Kikawada T
中科院分区：
文献类型：
--
作者：
Mitsumasu K;Kanamori Y;Fujita M;Iwata K;Tanaka D;Kikuta S;Watanabe M;Cornette R;Okuda T;Kikawada T
脱水昆虫的幼虫，Polypedilumvanderplanki，积累非常大量的海藻糖作为一个兼容的溶质干燥，但这种积累的分子机制尚不清楚。因此，我们分离了编码海藻糖代谢酶的基因，即合成步骤的海藻糖-6-磷酸合成酶（TPS）和海藻糖-6-磷酸磷酸酶（TPP），以及降解步骤的海藻糖酶（TREH）。尽管计算预测表明选择性剪接变体（PvTpsα/β）编码可能的功能基序，包括TPS的典型共有结构域和TPP的保守序列，但PvTpsα并不发挥TPP的活性，而仅发挥TPS的活性。相反，获得了表达TPP活性的不同基因（PvTpp）。以前的报道表明，昆虫TPS是一种特殊的双功能酶，同时控制TPS和TPP。在这篇文章中，我们提出，在昆虫的TPS和TPP活动可以归因于离散的基因。TRH直系同源物的翻译产物（PvTreh）肯定将海藻糖降解为葡萄糖。海藻糖合成丰富，与TPS和TPP活性增加和抑制TREH活性一致。这些结果表明，在脱水幼虫脱水诱导过程中观察到的海藻糖积累可以归因于海藻糖合成途径的激活和海藻糖水解的抑制。
Larvae of an anhydrobiotic insect, Polypedilum vanderplanki, accumulate very large amounts of trehalose as a compatible solute on desiccation, but the molecular mechanisms underlying this accumulation are unclear. We therefore isolated the genes coding for trehalose metabolism enzymes, i.e. trehalose-6-phosphate synthase (TPS) and trehalose-6-phosphate phosphatase (TPP) for the synthesis step, and trehalase (TREH) for the degradation step. Although computational prediction indicated that the alternative splicing variants (PvTpsα/β) obtained encoded probable functional motifs consisting of a typical consensus domain of TPS and a conserved sequence of TPP, PvTpsα did not exert activity as TPP, but only as TPS. Instead, a distinct gene (PvTpp) obtained expressed TPP activity. Previous reports have suggested that insect TPS is, exceptionally, a bifunctional enzyme governing both TPS and TPP. In this article, we propose that TPS and TPP activities in insects can be attributed to discrete genes. The translated product of the TREH ortholog (PvTreh) certainly degraded trehalose to glucose. Trehalose was synthesized abundantly, consistent with increased activities of TPS and TPP and suppressed TREH activity. These results show that trehalose accumulation observed during anhydrobiosis induction in desiccating larvae can be attributed to the activation of the trehalose synthetic pathway and to the depression of trehalose hydrolysis.
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DOI：
10.1186/1746-1448-4-18
发表时间：
2008-12-12
期刊：
Saline systems
影响因子：
--
作者：
Chung JS
通讯作者：
Chung JS
影响因子：
3.9
作者：
Goyal, K;Browne, JA;Tunnacliffe, A
通讯作者：
Tunnacliffe, A
影响因子：
3.4
作者：
Avonce N;Mendoza-Vargas A;Morett E;Iturriaga G
通讯作者：
Iturriaga G
影响因子：
2.2
作者：
HINTON, HE
通讯作者：
HINTON, HE
影响因子：
2.6
作者：
Mitsumasu, K;Azuma, M;Yaginuma, T
通讯作者：
Yaginuma, T