The arbitrium system controls prophage induction.
The arbitrium system controls prophage induction.
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DOI：
10.1016/j.cub.2021.08.072
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发表时间：
2021-11-22
期刊：
影响因子：
--
通讯作者：
Penadés JR
中科院分区：
文献类型：
--
作者：
Brady A;Quiles-Puchalt N;Gallego Del Sol F;Zamora-Caballero S;Felipe-Ruíz A;Val-Calvo J;Meijer WJJ;Marina A;Penadés JR
一些感染芽孢杆菌的噬菌体使用一种基于肽的通讯系统，称为arbitrium，以协调裂解-溶原性决定。在该系统中，噬菌体在裂解循环期间产生AimP肽。一旦被宿主细胞内化，AimP就与转录因子AimR结合，降低aimX表达并促进溶原性。虽然这些系统存在于各种移动的遗传元件中，但它们在噬菌体生命周期中的作用仅在噬菌体感染期间在噬菌体phi 3 T中表征。在这里，使用B. subtilis SPβ前噬菌体，我们表明arbitrium系统也是正常前噬菌体诱导所必需的。aimP基因的缺失增加了噬菌体的繁殖，虽然aimR的缺失显着减少了噬菌体诱导后产生的噬菌体颗粒的数量。此外，我们的研究结果表明，AimR参与了一个复杂的调控网络，并提出了两个新的球员在SPβ裂解溶原性决定系统，YopN和噬菌体阻遏YopR。重要的是，这些蛋白质编码在操纵子中，其功能在所有编码arbitrium系统的SPβ样蛋白中是保守的。最后，我们在arbitrium系统中获得了突变体，其表现与野生型（WT）噬菌体几乎相同，表明arbitrium系统在实验室中不是必需的，但可能对自然界中的噬菌体适应性有益。为了支持这一点，通过拥有功能性仲裁系统，SPβ噬菌体可以优化感染性颗粒的产生，同时也保留了原噬菌体诱导后存活的细胞数量，这是一种增加噬菌体在自然界中持久性的策略。枯草芽孢杆菌（B. subtilis）噬菌体诱导的仲裁系统（arbitrium system）在仲裁系统下游的操纵子参与控制溶原性。该操纵子在编码SPβ-类噬菌体的仲裁系统中功能保守。布雷迪等人表明该系统也是诱导宿主SOS反应激活后的常驻原噬菌体所必需的，并鉴定了参与控制裂解/溶原性循环的关键操纵子。
Some Bacillus-infecting bacteriophages use a peptide-based communication system, termed arbitrium, to coordinate the lysis-lysogeny decision. In this system, the phage produces AimP peptide during the lytic cycle. Once internalized by the host cell, AimP binds to the transcription factor AimR, reducing aimX expression and promoting lysogeny. Although these systems are present in a variety of mobile genetic elements, their role in the phage life cycle has only been characterized in phage phi3T during phage infection. Here, using the B. subtilis SPβ prophage, we show that the arbitrium system is also required for normal prophage induction. Deletion of the aimP gene increased phage reproduction, although the aimR deletion significantly reduced the number of phage particles produced after prophage induction. Moreover, our results indicated that AimR is involved in a complex network of regulation and brought forward two new players in the SPβ lysis-lysogeny decision system, YopN and the phage repressor YopR. Importantly, these proteins are encoded in an operon, the function of which is conserved across all SPβ-like phages encoding the arbitrium system. Finally, we obtained mutant phages in the arbitrium system, which behaved almost identically to the wild-type (WT) phage, indicating that the arbitrium system is not essential in the laboratory but is likely beneficial for phage fitness in nature. In support of this, by possessing a functional arbitrium system, the SPβ phage can optimize production of infective particles while also preserving the number of cells that survive after prophage induction, a strategy that increases phage persistence in nature. The arbitrium system controls prophage induction in B. subtilis An operon downstream of the arbitrium system is involved in controlling lysogeny The operon is functionally conserved in SPβ-like phages encoding arbitrium systems YopR acts as the phage repressor in SPβ Bacillus subtilis phages from the SPβ family use the arbitrium system to communicate during infection of the host. Brady et al. show that this system is also required for induction of the resident prophage after activation of the host SOS response and identify a key operon involved in the control of the lytic/lysogenic cycle.
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影响因子：
7.7
作者：
Xue KS;Hooper KA;Ollodart AR;Dingens AS;Bloom JD
通讯作者：
Bloom JD
影响因子：
14.9
作者：
Simossis VA;Heringa J
通讯作者：
Heringa J
DOI：
10.1073/pnas.81.5.1375
发表时间：
1984-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
LITTLE, JW
通讯作者：
LITTLE, JW
影响因子：
3
作者：
BURKHOLDER, PR;GILES, NH
通讯作者：
GILES, NH
影响因子：
64.8
作者：
Erez Z;Steinberger-Levy I;Shamir M;Doron S;Stokar-Avihail A;Peleg Y;Melamed S;Leavitt A;Savidor A;Albeck S;Amitai G;Sorek R
通讯作者：
Sorek R