Gene expression of brain nitric oxide synthase and soluble guanylyl cyclase in hypothalamus and medulla of two-kidney, one clip hypertensive rats.
Gene expression of brain nitric oxide synthase and soluble guanylyl cyclase in hypothalamus and medulla of two-kidney, one clip hypertensive rats.
复制标题
二肾一夹高血压大鼠下丘脑和延髓脑一氧化氮合酶和可溶性鸟苷酸环化酶基因表达
作者：
T. L. Krukoff;Frank Gehlen;Detlev Ganten;Jürgen Wagner
一氧化氮可作用于脑中的自主部位以调节动脉血压。我们的目标是确定是否基因表达的大脑亚型的一氧化氮合酶和β亚单位的可溶性鸟苷酸环化酶，一氧化氮的目标，在离散的自主脑区诱导高血压大鼠。采用两肾一夹模型诱导高血压，在夹闭左肾动脉后3周和6周进行测量。仅使用血压升高至少25 mm Hg的实验大鼠。从显微解剖的组织块中纯化总RNA，所述组织块含有下丘脑、背侧延髓、头侧腹外侧延髓和尾侧腹外侧延髓。一氧化氮合酶和鸟苷酸环化酶mRNA的变化进行半定量在每个区域通过使用逆转录-聚合酶链反应，其中已知浓度的两个基因的缺失突变体共扩增作为内部标准。与对照组相比，显着减少和增加一氧化氮合酶mRNA被发现在下丘脑（x 2.2）和尾侧延髓腹外侧（x 6.4），分别高血压大鼠夹闭后3周。这些变化在6周时在高血压大鼠中逆转;下丘脑中的水平增加（x 4.6），尾侧腹外侧髓质中的水平降低（x 5.5）。鸟苷酸环化酶表达的变化在两个时间点的一些但不是所有区域都与一氧化氮合酶的变化一致。（250字处删节）
Nitric oxide may act at autonomic sites in the brain to regulate arterial blood pressure. Our goal was to determine whether gene expressions of the brain isoform of nitric oxide synthase and of the beta subunit of soluble guanylyl cyclase, the target of nitric oxide, were altered in discrete autonomic brain regions after induction of hypertension in rats. The two-kidney, one clip model was used to induce hypertension, and measurements were made 3 and 6 weeks after the left renal artery was clipped. Only experimental rats with blood pressures elevated by at least 25 mm Hg were used. Total RNA was purified from microdissected tissue blocks containing hypothalamus, dorsal medulla, rostral ventrolateral medulla, and caudal ventrolateral medulla. Changes in nitric oxide synthase and guanylyl cyclase mRNA were semiquantified in each region by use of reverse transcription-polymerase chain reactions in which known concentrations of deletion mutants of the two genes were coamplified as internal standards. Compared with controls, significant decreases and increases in nitric oxide synthase mRNA were found in the hypothalamus (x 2.2) and caudal ventrolateral medulla (x 6.4), respectively, of hypertensive rats 3 weeks after clipping. These alterations were reversed in hypertensive rats at 6 weeks; levels increased (x 4.6) in the hypothalamus and decreased (x 5.5) in the caudal ventrolateral medulla. Changes in guanylyl cyclase expression paralleled those for nitric oxide synthase in some but not all areas at both time points.(ABSTRACT TRUNCATED AT 250 WORDS)
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影响因子：
20.1
作者：
I. Sakuma;H. Togashi;M. Yoshioka;Hideya Saito-;M. Yanagida;M. Tamura;Takeshi Kobayashi;H. Yasuda
通讯作者：
I. Sakuma;H. Togashi;M. Yoshioka;Hideya Saito-;M. Yanagida;M. Tamura;Takeshi Kobayashi;H. Yasuda
加压素和血管紧张素 II 对双肾 Goldblatt 高血压肾微循环动力学改变的相对贡献。
影响因子：
20.1
作者：
Ichikawa,I;Ferrone,RA;Duchin,KL;Manning,M;Dzau,VJ;Brenner,BM
通讯作者：
Brenner,BM
有证据表明一氧化氮可以集中刺激加压素的释放。
影响因子：
4.1
作者：
Ota,M;Crofton,JT;Festavan,GT;Share,L
通讯作者：
Share,L
编码来自大鼠肺的 70 千道尔顿可溶性鸟苷酸环化酶亚基的 cDNA 的分子克隆。
DOI：
10.1016/s0006-291x(88)80992-6
发表时间：
1988
影响因子：
3.1
作者：
Nakane,M;Saheki,S;Kuno,T;Ishii,K;Murad,F
通讯作者：
Murad,F
大鼠腮腺分泌颗粒成熟相关元素变化的微探针分析。
DOI：
10.1152/ajpcell.1991.261.6.c1033
发表时间：
1991
期刊：
The American journal of physiology
影响因子：
--
作者：
Wong,JG;Izutsu,KT;Robinovitch,MR;Iversen,JM;Cantino,ME;Johnson,DE
通讯作者：
Johnson,DE