Loss of social behaviours in populations of Pseudomonas aeruginosa infecting lungs of patients with cystic fibrosis.
Loss of social behaviours in populations of Pseudomonas aeruginosa infecting lungs of patients with cystic fibrosis.
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DOI：
10.1371/journal.pone.0083124
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Griffin AS
中科院分区：
文献类型：
--
作者：
Jiricny N;Molin S;Foster K;Diggle SP;Scanlan PD;Ghoul M;Johansen HK;Santorelli LA;Popat R;West SA;Griffin AS
铜绿假单胞菌是一种机会性细菌性病原体，可引起囊性纤维化患者持续且经常致命的肺部感染。从慢性感染中分离出来的菌株在一系列特征上不同于实验室和环境菌株，这被广泛解释为适应肺部环境的结果。通常，慢性菌株携带的全球调节因子突变可能会影响社会特征的表达减少，这增加了合作与自私、欺骗菌株之间的竞争动力可能也会推动铜绿假单胞菌感染的变化。我们比较了铜绿假单胞菌（P. aeruginosa）分离株的合作性状——生物膜形成、外产物分泌和群体感应（QS）——的表达，这些分离株根据临床信息估计在肺部停留了不同的时间。随着感染时间的延长，参与营养获取的所有三种外显产物的产量都显著减少，并且四个QS信号分子的模式与lasR突变随时间的积累一致，这些突变已知会破坏细胞对QS信号的反应能力。Pyocyanin的产生，以及生物膜中细胞相对于液体培养中活动的、自由活的细胞的比例没有变化。总的来说，我们的研究结果证实，社交行为的丧失与在肺部停留的时间是一致的趋势，并表明社交动态可能与理解铜绿假单胞菌在肺部感染中的行为有关。
Pseudomonas aeruginosa, is an opportunistic, bacterial pathogen causing persistent and frequently fatal infections of the lung in patients with cystic fibrosis. Isolates from chronic infections differ from laboratory and environmental strains in a range of traits and this is widely interpreted as the result of adaptation to the lung environment. Typically, chronic strains carry mutations in global regulation factors that could effect reduced expression of social traits, raising the possibility that competitive dynamics between cooperative and selfish, cheating strains could also drive changes in P. aeruginosa infections. We compared the expression of cooperative traits - biofilm formation, secretion of exo-products and quorum sensing (QS) - in P. aeruginosa isolates that were estimated to have spent different lengths of time in the lung based on clinical information. All three exo-products involved in nutrient acquisition were produced in significantly smaller quantities with increased duration of infection, and patterns across four QS signal molecules were consistent with accumulation over time of mutations in lasR, which are known to disrupt the ability of cells to respond to QS signal. Pyocyanin production, and the proportion of cells in biofilm relative to motile, free-living cells in liquid culture, did not change. Overall, our results confirm that the loss of social behaviour is a consistent trend with time spent in the lung and suggest that social dynamics are potentially relevant to understanding the behaviour of P. aeruginosa in lung infections.
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影响因子：
3.6
作者：
Diggle, SP;Winzer, K;Williams, P
通讯作者：
Williams, P
DOI：
10.1099/00221287-13-3-572
发表时间：
1955-01-01
期刊：
JOURNAL OF GENERAL MICROBIOLOGY
影响因子：
--
作者：
HOLLOWAY, BW
通讯作者：
HOLLOWAY, BW
DOI：
10.1007/bf00281792
发表时间：
1994-02-01
期刊：
MOLECULAR & GENERAL GENETICS
影响因子：
--
作者：
AYORA, S;GOTZ, F
通讯作者：
GOTZ, F
影响因子：
5.2
作者：
Carlsson, Malin;Shukla, Swati;Hellmark, Thomas
通讯作者：
Hellmark, Thomas
影响因子：
3.1
作者：
HANCOCK, REW;MUTHARIA, LM;PIER, GB
通讯作者：
PIER, GB