Hydrocarbon biodegradation and transcriptome responses of cellulase, peroxidase, and laccase encoding genes inhabiting rhizospheric fungal isolates.
Hydrocarbon biodegradation and transcriptome responses of cellulase, peroxidase, and laccase encoding genes inhabiting rhizospheric fungal isolates.
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DOI：
10.1016/j.sjbs.2021.01.009
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发表时间：
2021-04
影响因子：
4.4
通讯作者：
Mahmoud MA
中科院分区：
文献类型：
--
作者：
Al-Zaban MI;AlHarbi MA;Mahmoud MA
通过利用待处理的污染环境中的本地微生物，生物修复可以是一种成功的策略。应用PCR和RT-PCR分子技术，通过木质素过氧化物酶（LiP）、漆酶（LaC）、锰过氧化物酶（MNP）和纤维素酶（Cx）等木质素分解酶的推定基因来研究真菌分离株的进化，以响应碳氢化合物对环境的污染。在这项研究中，根际真菌菌株的分离，分子鉴定，原油耐受性，和酶分泌。从枣树根际分离到3个真菌分离物，并通过ITS核糖体RNA（rRNA）测序对其进行了形态学和分子生物学特征分析。通过NCBI的BLAST工具，将分离到的菌株鉴定为黄曲霉（Aspergillus flavus）AF 15、哈茨木霉（Trichoderma harzianum）TH 07和茄病镰刀菌（Fusarium solani）FS 12。所有真菌菌株对原油表现出很高的耐受性，并在最高浓度（20%）下以各种反应存活。根据菌株的剂量抑制反应百分比（DIRP），黄曲霉AF 15和哈茨木霉TH 07表现出良好的耐油降解能力。A.黄曲霉产Cx、LaC、LiP和MnP的能力较强，最大产气量为83.7 ~ 96.3mL。在分子水平上，测试了木质素分解酶cbh（cbhI.1，cbhI.1，cbhII）lcc，lig（1，2，4和6）和mnp的9个基因的存在和表达（分别通过PCR和RT-PCR）。PCR结果表明，所有菌株都含有所有9个基因的检查，无论能力的酶生产概况，所以9个基因的存在响应与产酶能力无关。基因表达谱分析表明，黄曲霉AF 15的lig和mnp基因过表达，茄病镰刀菌FS 12的lcc基因表达较弱，哈茨木霉TH 07的mnp和lcc基因表达较弱。该基因的转录能力导致真菌分离物的酶分泌增加。真菌是石油污染治理中的重要微生物。它们具有很强的生物修复能力，这与它们产生的这些催化酶的多样性有关。
By using the indigenous micro-organisms of the polluted environment to be treated, bioremediation can be a successful strategy. PCR and RT-PCR molecular techniques were applied to examine the evolution of fungal isolates through putative genes f ligninolytic enzymes like lignin peroxidase (LiP), laccase (LaC), manganese peroxidase (MnP), and cellulase (Cx) as a response to polluting of the environment by hydrocarbons. In this study, isolation of rhizospheric fungal isolates, molecular identification, crude oil tolerance, and enzyme excretions were demonstrated. From the date palm rhizosphere, 3 fungal isolates were isolated and characterized morphologically and molecularly by ITS ribosomal RNA (rRNA) sequencing. The isolates were identified as Aspergillus flavus AF15, Trichoderma harzianum TH07, and Fusarium solani FS12 through using the BLAST tool in NCBI. All fungal isolates showed high tolerance to crude oil and survived with various responses at the highest concentration (20%). Aspergillus flavus AF15 and Trichoderma harzianum TH07 demonstrated promising oil-degrading tolerance ability based on the dose inhibition response percentage (DIRP) of the fungal isolates. A. flavus had a powerful capacity to production Cx, LaC, LiP and MnP with a range from 83.7 to 96.3 mL. Molecularly, nine genes of the ligninolytic enzymes, cbh (cbhI.1, cbhI.1, cbhII) lcc, lig (1, 2, 4 and 6) and mnp were tested for presence and expression (by PCR and RT-PCR, respectively). PCR showed that all isolates contained all the nine genes examined, regardless of capacity to enzymes production profiles, so the presence responses of nine genes did not correlate with enzymes-production ability. Gene expression analysis shows a more diverse pattern for tested isolates for example, Aspergillus flavus AF15 had over-expression of lig and mnp genes, Fusarium solani FS12 have a weak signal with lcc gene while, Trichoderma harzianum TH07 showed moderate expression of mnp and lcc genes. The power of the transcription of the gene leads to increased enzyme secretion by fungal isolates. Fungi are important microorganisms in the clean-up of petroleum pollution. They have bioremediation highly potency that is related to their diverse production of these catalytic enzymes.
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影响因子：
3.1
作者：
Belen Florez, Ana;Alvarez-Martin, Pablo;Mayo, Baltasar
通讯作者：
Mayo, Baltasar
影响因子：
4.1
作者：
Chan, Sidney Man Ngai;Luan, Tiangang;Tam, Nora Fung Yee
通讯作者：
Tam, Nora Fung Yee
影响因子：
6.1
作者：
Del Pilar Castillo, Maria;Torstensson, Lennart
通讯作者：
Torstensson, Lennart
影响因子：
4.4
作者：
BRODA, P;BIRCH, PRJ;SIMS, PFG
通讯作者：
SIMS, PFG
影响因子：
4.2
作者：
Hamamura, Natsuko;Ward, David M.;Inskeep, William P.
通讯作者：
Inskeep, William P.