Nanoparticle diffraction gratings for DNA detection on photopatterned glass substrates.
Nanoparticle diffraction gratings for DNA detection on photopatterned glass substrates.
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DOI：
10.1116/1.2994689
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发表时间：
2008-09
期刊：
影响因子：
2.1
通讯作者：
Corn RM
中科院分区：
文献类型：
--
作者：
Sendroiu IE;Corn RM
利用DNA修饰的纳米颗粒附着在玻璃基底上的DNA单层光栅上，建立了一种非原位纳米颗粒DNA检测方法。该测定利用单链DNA（ssDNA）靶分子与附着于胺反应性玻璃表面的胺修饰的DNA寡核苷酸和附着于13 nm金纳米颗粒的硫醇修饰的DNA寡核苷酸的同时杂交。表面等离子体共振成像（SPRI）测量用于表征在测定中采用的两个顺序的杂交吸附过程，和荧光显微镜用于表征通过胺反应性载玻片的双染色形成的DNA单层光栅。利用非相干白色光源和以600 nm为中心的10 nm带通滤波器的一级衍射测量提供了低至10 pM浓度的靶ssDNA的定量测量。采用HeNe激光器和雪崩光电二极管的四阶衍射测量用于检测来自10 μL浓度低至10 fM的溶液的靶ssDNA吸附，对应于60，000个靶DNA分子。这种简单而灵敏的基于光栅的纳米颗粒DNA检测分析应该直接适用于遗传筛查、mRNA表达分析和microRNA分析。
An ex situ nanoparticle DNA detection assay utilizing DNA modified nanoparticles attached to DNA monolayer gratings on glass substrates is developed. The assay utilizes the simultaneous hybridization of a single stranded DNA (ssDNA) target molecule to both an amine-modified DNA oligonucleotide attached to an amine-reactive glass surface, and a thiol-modified DNA oligonucleotide attached to a 13 nm gold nanoparticle. Surface plasmon resonance imaging (SPRI) measurements are used to characterize the two sequential hybridization adsorption processes employed in the assay, and fluorescence microscopy is used to characterize the formation of DNA monolayer gratings via the photopatterning of the amine-reactive glass slides. First order diffraction measurements utilizing incoherent white light source and a 10 nm bandpass filter centered at 600 nm provided quantitative measurements of target ssDNA down to a concentration of 10 pM. Fourth order diffraction measurements employing a HeNe laser and avalanche photodiode were used to detect target ssDNA adsorption from 10 μL of a solution with a concentration as low as 10 fM, corresponding to 60,000 target DNA molecules. This simple yet sensitive grating-based nanoparticle DNA detection assay should be directly applicable for genetic screening, mRNA expression assays, and microRNA profiling.
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DOI：
10.1126/science.1139253
发表时间：
2007-04-27
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Rodriguez A;Vigorito E;Clare S;Warren MV;Couttet P;Soond DR;van Dongen S;Grocock RJ;Das PP;Miska EA;Vetrie D;Okkenhaug K;Enright AJ;Dougan G;Turner M;Bradley A
通讯作者：
Bradley A
影响因子：
3.9
作者：
Goodrich, GP;Helfrich, MR;Keating, CD
通讯作者：
Keating, CD
影响因子：
15
作者：
He, L;Musick, MD;Keating, CD
通讯作者：
Keating, CD
影响因子：
16.6
作者：
Arenz, Christoph
通讯作者：
Arenz, Christoph
影响因子：
7.4
作者：
Nelson, BP;Grimsrud, TE;Corn, RM
通讯作者：
Corn, RM