Establishment of the ac electrokinetic elongation mechanism of DNA by three-dimensional fluorescent imaging
Establishment of the ac electrokinetic elongation mechanism of DNA by three-dimensional fluorescent imaging
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三维荧光成像建立DNA交流电动延伸机制
DOI：
10.1063/1.2188587
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发表时间：
2006
影响因子：
4
通讯作者：
C. Kaminski
中科院分区：
文献类型：
--
作者：
C. Wälti;P. Tosch;A. Davies;W. A. Germishuizen;C. Kaminski
我们报告的三维成像测量使用共聚焦显微镜的荧光标记的脱氧核糖核酸（DNA）链进行强交流电场。的DNA分子共价拴在一端的金微电极和观察到的伸长模式进行比较，从数值模拟和先前确定的流体流动模式获得的电场线。我们表明，主要贡献的伸长率源于交流电动扭矩，由电场诱导的流体流动提供的一个小的偏置力的补充，我们提供的证据表明，由于电极的几何形状的结果从偏置力的符号变化所观察到的限制伸长率。
We report three-dimensional imaging measurements using confocal microscopy of fluorescently labelled deoxyribonucleic acid (DNA) strands subjected to strong ac electric fields. The DNA molecules are covalently tethered by one end to gold microelectrodes and the observed elongation patterns are compared with the electric field lines obtained from numerical simulations and with previously determined fluid flow patterns. We demonstrate that the major contribution to the elongation stems from the ac electrokinetic torque, supplemented by a small bias force provided by the electric-field-induced fluid flow, and we provide evidence that the observed restricted elongation owing to the geometries of the electrodes results from a sign change in the bias force.
影响因子：
14.9
作者：
Ralf M. Zimmermann;Edward C. Cox
通讯作者：
Ralf M. Zimmermann;Edward C. Cox