In vivo cyclic compression causes cartilage degeneration and subchondral bone changes in mouse tibiae.
In vivo cyclic compression causes cartilage degeneration and subchondral bone changes in mouse tibiae.
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DOI：
10.1002/art.37906
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发表时间：
2013-06
影响因子：
--
通讯作者：
van der Meulen, Marjolein C. H.
中科院分区：
文献类型：
--
作者：
Ko, Frank C.;Dragomir, Cecilia;Plumb, Darren A.;Goldring, Steven R.;Wright, Timothy M.;Goldring, Mary B.;van der Meulen, Marjolein C. H.
关节力学负荷环境的改变对关节软骨和软骨下骨既有有利的影响，也有不利的影响，从而影响骨关节炎的发展。我们使用活体胫骨负荷模型来研究软骨和骨对机械负荷的适应性反应，并评估负荷水平和持续时间的影响。对成年(26周龄)C57BL/6雄性小鼠经膝关节对左侧胫骨施加4.5和9.0N的峰值载荷，分别持续1、2和6周。幼龄(10周龄)小鼠仅使用9.0N负荷。通过组织学和显微CT分析关节软骨和软骨下骨的变化。负荷促进了两个年龄组的软骨损伤，损伤严重程度随负荷时间的延长而增加。幼年组小鼠干骺端骨量增加，但成年组小鼠不增加，而年轻和成年组小鼠的骨痂松质骨量随着负荷的增加而减少。两个年龄组胫骨后平台关节软骨厚度减少，软骨下皮质骨厚度增加。两个年龄组的小鼠在9.0N的负荷下都在胫骨平台形成了关节周围的骨赘，但在4.5N的峰值负荷下成年小鼠没有骨赘的形成。这种非侵入性加载模型可以解剖软骨和骨骼的时间和地形变化，并将能够研究针对关节生物力学或促进骨关节炎发生和发展的生物事件的治疗干预的有效性。
Alterations in the mechanical loading environment in joints may have both beneficial and detrimental effects on articular cartilage and subchondral bone and subsequently influence the development of osteoarthritis (OA). We used an in vivo tibial loading model to investigate the adaptive responses of cartilage and bone to mechanical loading and to assess the influence of load level and duration. We applied cyclic compression of 4.5 and 9.0N peak loads to the left tibia via the knee joint of adult (26-week-old) C57Bl/6 male mice for 1, 2, and 6 weeks. Only 9.0N loading was utilized in young (10-week-old) mice. The changes in articular cartilage and subchondral bone were analyzed by histology and microcomputed tomography. Loading promoted cartilage damage in both age groups, with increased damage severity dependent upon the duration of loading. Metaphyseal bone mass increased in the young mice, but not in the adult mice, whereas epiphyseal cancellous bone mass decreased with loading in both young and adult mice. Articular cartilage thickness decreased, and subchondral cortical bone thickness increased in the posterior tibial plateau in both age groups. Both age groups developed periarticular osteophytes at the tibial plateau in response to the 9.0N load, but no osteophyte formation occurred in adult mice subjected to 4.5N peak loading. This non-invasive loading model permits dissection of temporal and topographical changes in cartilage and bone and will enable investigation of the efficacy of treatment interventions targeting joint biomechanics or biological events that promote OA onset and progression.
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影响因子：
--
作者：
Lawrence, Reva C.;Felson, David T.;Wolfe, Frederick
通讯作者：
Wolfe, Frederick
影响因子：
7
作者：
Botter, S. M.;Glasson, S. S.;van Osch, G. J. V. M.
通讯作者：
van Osch, G. J. V. M.
影响因子：
7
作者：
Christiansen, B. A.;Anderson, M. J.;Haudenschild, D. R.
通讯作者：
Haudenschild, D. R.
影响因子：
6.2
作者：
Intema, Femke;Sniekers, Yvonne H.;Mastbergen, Simon C.
通讯作者：
Mastbergen, Simon C.
影响因子：
3.9
作者：
JEFFREY, JE;GREGORY, DW;ASPDEN, RM
通讯作者：
ASPDEN, RM